【摘要】 研究了绿豆提取液对细菌内毒素的清除作用,利用终点显色基质法测定内毒素的含量。结果表明,绿豆提取液在体外具有抗内毒素的作用,并以内毒素的清除率为标准,探讨了不同提取因素对提取效果的影响,最终确定提取条件为乙醇浓度70%,温度60℃,提取时间2h,料液比1∶20,提取物中抗内毒素有效成分含量最高。最后通过两相萃取分离,发现绿豆中抗内毒素成分主要集中在正丁醇提取物中。更多还原

【Abstract】 The clearing effect of extraction from mung bean on the bacterial endotoxin was investigated in vitro.The results indicated that extraction from mung bean could clear the bacterial endotoxin in vitro with displaying color in the end.And the influences of different factors on extracted efficiency were discussed.The conditions of 60℃,70% ethanol,solid-liquid ratio of 1∶20,extracting for 2h were optimized.And it was found that the effective components existed in the extraction of chloroform through extracting in the two-phase.更多还原