【摘要】 以金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌、啤酒酵母、黑曲霉为受试菌对两种大型海藻提取物进行抑菌试验,采用打孔法对提取物的抗菌活性进行研究,并研究了加热、酸碱处理对抑菌效果的影响。结果表明,裙带菜、海白菜的水、甲醇提取物对枯草芽孢杆菌、金黄色葡萄球菌有抑制作用,对大肠杆菌、黑曲霉及啤酒酵母均无抑制作用。海白菜的乙醇提取物对枯草芽孢杆菌、金黄色葡萄球菌有抑菌效果。海白菜甲醇提取物在pH7时对金黄色葡萄球菌的抑制效果较好,抑菌圈宽度达到3.5mm左右,对枯草芽孢杆菌的最大抑菌效果的pH为6,抑菌圈宽度为2.8mm左右。裙带菜甲醇提取物经100℃处理30min后,对金黄色葡萄球菌的抑菌圈宽度达到了7.5mm～8.0mm左右,对枯草芽孢杆菌的抑菌圈宽度达到了6.0mm～6.5mm左右,海白菜甲醇提取物经100℃加热处理后对金黄色葡萄球菌的抑菌圈宽度为3.0mm～3.5mm左右,对枯草芽孢杆菌的抑菌圈宽度为3.8mm～4.5mm左右,均高于对照值。更多还原

【Abstract】 The antiseptic effect of extraction substance about two large seaweed was determined with Staphylococcus aureus,Bacillus subtilis,Escherichia coli,S.cerevisiae and Aspergillus niger as the testing microbe.antiseptic activity was research by Drilling method,the effects of heating and acid-alkali treatment on the inhibitory were also studied.The results show that the water,methanol extracts of Undarzia pinnatifida Suringer and Ulva pertusa had inhibitory effect on Staphylococcus aureus and Bacillus subtilis,the ethanol extract of Ulva pertusa had inhibitory effect on Bacillus subtilis and Staphylococcus aureus.The methanol extract of Ulva pertus had better inhibitory effect on Staphylococcus aureus when pH was 7,the width of inhibition zone was 3.5 mm.The methanol extract of Ulva pertus had inhibitory effect on Bacillus subtilis when pH was 6,the width of inhibition zone was 2.8 mm.After the methanol extract of Undariza pinnatifida Suringer was treated with heating at 100 ℃ for 30 min,the width of inhibition zone on Staphylococcus aureus was 7.5 mm～8.0 mm,the width of inhibition zone on Bacillus subtilis was6.0 mm～6.5 mm.After the methanol extract of Ulva pertus was treated with heating at 100 ℃,the width of inhibition zone on Staphylococcus aureus was 3.0 mm～3.5 mm,the width of inhibition zone on Bacillus subtilis was 3.8 mm～4.5 mm,these were higher than contral value.However,there is no significant change in inhibitory effect with acid-alkali treatment.更多还原