【摘要】 研究从罗非鱼鱼鳞中提取胶原的最佳工艺。研究表明,鱼鳞先经0.4 M盐酸(料液比1∶20)脱盐1 h,再经0.5 M EDTA(料液比1∶20)脱盐2 h,除钙率达100%。再用0.1 M氢氧化钠处理12 h,去除蛋白率达6.8%。最后用添加了1%胃蛋白酶的0.5 M乙酸提取,提取时间96 h,每48 h更换一次提取液,提取温度10℃,料液比1∶10,提取率达79.2%。经透析、浓缩、冻干后得到白色胶原粉末,胶原纯度达到92%。更多还原

【Abstract】 The conditions for extraction of collagen from tilapia scale were optimized in the paper.Scale should be decalcified and removed impurity before collagen extraction.The scale is decalcified by HCl with 0.4M concentration and 1∶20(W/V) ratio of scale to HCl for 1 h and then by EDTA with 0.5M concentration and 1∶20(W/V) ratio for 2 h with 100% decalcification.Then the impurity is removed by NaOH with 0.1M concentration and 1∶20(W/V) ratio for 12 h with 6.8% removing rate.0.5 M acetic acid within 1% pepsin and 1∶10(W/V) ratio is adopted for extraction in 10℃ for 96 h with 79.2% collagen extraction rate.White collagen power with 92% purification rate is obtained through dialysis,condensation and lyophilization.更多还原