【摘要】 目的观察矽尘接触者和矽肺患者外周血单核细胞核转录因子-κB(NF-κB)水平的变化,探讨NF-κB在矽肺发生发展中的作用。方法采用ELISA法对100名不接触粉尘的对照人群,200名接触矽尘1年以上的接尘工人,32名矽尘作业观察对象(原诊断0+患者)及79例矽肺患者,检测其外周血单核细胞胞核蛋白中的NF-κB水平。结果矽肺组NF-κB水平较对照组和接尘组明显升高,接尘组NF-κB水平较对照组明显升高,差异均有统计学意义;在不同期别矽肺患者中NF-κB水平随矽肺期别升高也有升高的趋势。多元回归分析也表明NF-κB水平与接尘情况和矽肺的病情有关。结论接尘组和矽肺组外周血单核细胞NF-κB均有升高,此种升高除与矽肺的发病有关外,与矽肺的严重程度也有关,提示对外周血单核细胞NF-κB水平的监测可能成为矽肺发病预测、预后评估的生物标志物,而适当干预NF-κB的活化可能是矽肺防治新的干预靶点。更多还原

【Abstract】 Objective To explore the role of nuclear factor-κB(NF-κB)in the development of silicosis through investigating the change of NF-κB level in peripheral monocytes of silica dust exposed workers and silicosis patients.Methods One hundred workers never exposed to silica dust were selected as control group,200 workers exposed to silica dust for more than 1 year as exposed group,32 suspected silicosis workers as observing group,and 79 silicosis patients as silicosis group.Levels of NF-κB protein in the peripheral monocytes of all the subjects were determined by ELISA method.Results The NFκB level in silicosis group was significantly higher than those of control group and exposed group,and the level in exposed group was significantly higher than that of control group,meanwhile,the level of NF-κB in silicosis group showed some increase along with the silicosis stage;the results of multiple regression analysis also showed that NF-κB level was correlated with the stage of silicosis.Conclusion Data from this study showed that the level of NF-κB in peripheral monocytes was increased in silica dust exposed group and silicosis group,which was not only correlated with the occurrence but also the severity of silicosis,suggested that NF-κB may become a good biomarker for prediction and prognosis of silicosis,and block the activation of NF-κB may be the new intervention target for prevention and treatment of silicosis.更多还原