【摘要】 探究布地奈德(budesonide,BUD)对人气道上皮9HTEo-细胞增殖的影响及机制,利用CCK-8法检测BUD对9HTEo-细胞增殖的影响,荧光显微镜观察经BUD处理后9HTEo-细胞形态的改变,流式细胞术检测细胞周期分布变化,Western blot检测p27kip1蛋白表达.CCK-8法结果显示3~10μmol/L BUD对细胞具有显著的抑制作用(P<0.01);荧光显微镜下,1~10μmol/L BUD处理后细胞可见明显的增殖受抑、凋亡形态;流式细胞术检测G0/G1期细胞随着BUD浓度的增加而增多,S期细胞逐渐减少;Western blot检测结果提示BUD能使p27kip1表达增加.由此可见,BUD对人气道上皮细胞株9HTEo-具有显著增殖抑制作用,其作用与提高p27kip1蛋白的表达,将细胞阻滞在G0/G1期有关.更多还原

【Abstract】 To investigate the effects and possible mechanisms of budesonide(BUD) on proliferation of human airway epithelial 9HTEo-cells,cell proliferation was determined by Cell Counting Kit-8 assay.Fluorescence microscopy was used to observe the morphological changes of 9HTEo-cells induced by BUD.Cell cycle was analyzed by flow cytometry(FCM),and the expression of p27kip1 was detected by Western blot method.The results showed that BUD could significantly inhibit the growth of 9HTEo-cells(P<0.01) at the concentration 3~10 μmol/L.Marked morphological changes of 9HTEo-cells were found clearly using Hoechst 33258 staining.With the gradual increase of BUD concentration,the G0/G1 cells increased by degrees,while the S-phase cells decreased gradually,and Western blot results showed that the expression of p27kip1 of 9HTEo-cells was up-regulated.These experiments demonstrated that BUD has a significantly effect on inhibiting proliferation of 9HTEo-cells.Moreover,blocking 9HTEo-cells at the G0/G1 phase by enhancing the expres-sion of p27kip1 protein may be one of the mechanisms of BUD inhibiting 9HTEo-proliferation.更多还原