【摘要】 目的:通过制备完全性脊髓损伤(SCI)成年SD大鼠模型,研究生长相关蛋白(GAP-43)治疗大鼠SCI后胶质原纤维酸性蛋白(GFAP)的变化,探讨GAP-43在再生修复中的作用,为临床治疗提供实验参考。方法:咬除T7-T8棘突及相应椎板,用剪刀将脊髓完全横断,制成SCI模型。雌性8周龄SD大鼠75只,随机分为三组:GAP-43抗体组、GAP-43抗原组、对照组,每组25只。使用直接注射法将GAP-43抗原和GAP-43多克隆抗体分别注入抗原组和抗体组的大鼠脊髓的断端,观察各组大鼠肢体功能的恢复情况,用BBB评分法进行不同时段的行为学评分、免疫组化染色及图像分析方法观察GFAP的表达变化,并对其进行相关性分析。结果:对照组大鼠在不同时间段的行为学评分最低,抗原组评分最高;抗原组GFAP阳性细胞显著增多,而抗体组晚期则显著减少。结论:GAP-43可促进星形胶质细胞增生,而GAP-43抗体对星形胶质细胞的增生则表现为抑制作用。本实验结果表明GAP-43对脊髓损伤具有较好的治疗作用。更多还原

【Abstract】 Objective: In order to provide important data for neuronal factor growth associated protein(GAP-43) therapy after spinal cord injury.To study the changes of glial fibrillary acidic protein(GFAP) in complete spinal cord injury(SCI) model.Methods: After the spinous process and corresponding vertebral laminae were removed,the spinal cord was cut by using scissors to prepare the complete transversal spinal model.Seventy-five female SD rat at aged 8 weeks were divided into three groups at random,including GAP-43 antibody therapy group,GAP-43 antigen therapy group and control group with 25 rats in each group.After GAP-43 antibody or antigen had been injected into the sectioned sites of the SCI,the behavioral scores of the rats were evaluated at different time Then immunohistochemical staining was used to examine the expressional change of GFAP in the damaged spinal segments.Results: The score of rat in the experiment group was increased compared with the control group at different control time.The expression of GFAP was increased significantly in GAP-43 antigen therapy group.But in GAP-43 antibody therapy group GFAP expression was reduced significantly in the later time.Conclusion: GAP-43 could promote the growth of astrocyte,but the effect of GAP-43 antibody was opposite.The present results indicate that GAP-43 has the potent neuroprotective effect for SCI.更多还原