【摘要】 目的:探讨胶质细胞源性神经营养因子(GDNF)作用下,中脑源神经干细胞(mNSCs)在帕金森病(PD)大鼠模型纹状体移植区表达垂体同源盒家族因子3(Pitx3)、孤儿核受体相关因子1(Nurr1)和酪氨酸羟化酶(TH)的变化。方法:mNSCs单独移植组、mNSCs+GDNF移植组和生理盐水假手术组的PD大鼠模型,移植4周和8周后采用免疫荧光和Western Blot方法检测移植区Pitx3、Nurr1和TH的表达。结果:(1)mNSCs单独移植组和mNSCs联合GDNF移植治疗组在移植后4周、8周移植区均有Pitx3,Nurr1和TH表达,mNSCs单独移植组较少表达Pitx3,Nurr1;(2)Western Blot检测显示联合移植组移植区Pitx3、Nurr1和TH表达量高于单独移植组,其中联合移植组4周表达量最高(P<0.05)。结论:GDNF有促进PD大鼠模型脑内移植的mNSCs表达Pitx3、Nurr1和TH的作用。更多还原

【Abstract】 Objective:To explore the expressional change of pituitary homeobox 3(Pitx3),nuclear receptor related factor 1(Nurr1) and tyrosine hydroxylase(TH) in corpus striatum transplanted midbrain-derived neural stem cells(mNSCs) of Parkinson’s disease(PD) model of rats in vivo with the contribution of the glial cell line-derived neurotrophic factor(GDNF).Methods:The protein levels of Pitx3,Nurr1 and TH in the transplanted regions of PD model of rats of the single mNSCs transplanted groups,mNSCs combinated GDNF transplanted groups and sham operation groups have been detected by Western Blot or immunofluorescence histochemical methods after four or eight weeks,respectively.Results:(1) After four or eight weeks,the single mNSCs transplanted groups and added GDNF transplanted groups all expressed Pitx3-,Nurr1-and TH-immunoreactivities in transplanted regions.Compared with added GDNF groups,the single mNSCs transplanted groups hardly express Pitx3 and Nurr1;(2) Quantitatively measured Western Blot analysis of Pitx3,Nurr1 and TH expression in the transplanted regions of the mNSCs combinated GDNF transplanted groups showed that the expression is higher than the single mNSCs transplanted groups,the maximum expression in the mNSCs and GDNF co-transplanted groups after transplanted four weeks(P<0.05).Conclusion:GDNF can promote the expression of Pitx3,Nurr1 and TH in transplanted mNSCs of PD model of rats in vivo.更多还原