【摘要】 为检测新疆维吾尔族宫颈癌中HPV16变异情况,并建立HPV16系统生成树。选用HPV16感染的维吾尔族宫颈癌40例,德国HPV16标准株做阳性对照,PCR扩增HPV16E6后纯化并双向测序;应用Lasergene、DNAstar、clustal 1.83、Mega 4.1软件对HPV16E6序列进行比对分析,并最终生成系统生成树。结果显示:在40例维吾尔族宫颈癌中发现HPV16变异位点4个:350nt(T→G),310nt(T→G),295nt(T→G),135nt(A→C);变异率最高位点为350nt(71%,20/28);相应氨基酸变化为L→V,F→L,D→E,K→T。通过系统生成树发现,本组样本全为欧洲变异体;HPV16E6 350位点变异与分化程度及年龄分组分别作比较(P>0.05),两者之间无统计学差异。由此可知,从系统生成树角度分析,维吾尔族宫颈癌中HPV16变异体为欧洲变异体;突变率最高位点为高危的350位点,其次为310、295、135位点,这些变异位点与维吾尔族宫颈癌的相关性有待进一步研究。更多还原

【Abstract】 Objective to detected the variants of HPV16 and structured HPV 16 variants’ phylogenetic tree of Uighur’s cervical squamous cell carcinoma(CSCC).Methods:selecting 40 cases of Uighur’s CSCC,which had HPV16 infected;Using HPV16 standard strain as positive control and amplificate HPV16 E6 genes,which were sequenced,blasted and analyzed by Lasergene,DNAstar.Finally,phylogenetic tree of HPV16 was drawed by using clustal 1.83、Mega4.1 program;all the statistical dates were analyzied by SPSS17.0 software package.4 variant sites of HPV16 were found in 40 cases of Uighur’s CSCC:350nt(T→G),310nt(T→G),295nt(T→G),135nt(A→C);the most frequently variant site was 350nt,(71%,20/28);The variation of corresponding amino acids were L→V,F→L,D→E,K→T.Following by phylogenetic tree was structured,we found all of the cases in this group were E variant;Compared HPV 350 site’s variant rate with age,differentiation degree,their relations were no statistical significance(P>0.05).By the aspect of phylogenetic tree,the variant of HPV16 was E variant in Uighur’s CSCC;the most frequently variant sites were 350,the follow sites were 310,295,135 sites.It need further research to identify if these variant sites and variant brach were related with CSCC.更多还原