【摘要】 目的了解青岛市乙型肝炎患者乙型肝炎病毒(HBV)基因变异特征,探讨HBV YMDD变异与基因分型、DNA载量及临床分型的关系。方法应用实时荧光聚合酶链反应(PCR)技术对820例乙肝患者血清进行基因变异检测,HBV DNA定量监测及基因分型检测。结果 820例HBV感染者中检出YMDD变异154例,变异率18.78%;各基因型间YMDD变异的差异有统计学意义,P<0.05;患者HBeAg阳性与否与YMDD变异差异有统计学意义(P<0.05),HBeAg阳性者更易发生变异;HBV感染者临床分型各组间YMDD变异的差异有统计学意义(P<0.05),其中以LC(36/113)、CHB(97/368)为主;病毒变异在性别、年龄及DNA载量各组间的差异均无统计学意义(P>0.05)。结论 HBV YMDD变异的监测对乙肝患者的治疗、病情预测和转归都有重要临床意义。更多还原

【Abstract】 Objective To investigate hepatitis B virus(HBV) variation features in the patients with hepatitis B in Qingdao,and the relationship of HBV YMDD variation with genotyping,DNA load and clinical classification.Methods The YMDD variation,DNA load and genotyping were determined in 820 patients with hepatitis B by real-time fluorescence quantitative polymerase chain reaction(PCR).Results The YMDD variations were determined in 154 patients with hepatitis B,and the variation rate was 18.78%.The YMDD variations between the different genotypes were statistically different(P< 0.05).HBeAg positive or not was statistically significant with YMDD variation(P<0.05),and the variation happened more easily in the patients with HBeAg positive.YMDD variation between the different HBV infection clinical classification had statistical significance(P<0.05).The LC(36/113) and CHB(97/368) were the most.The differences between sex,age and DNA load groups were not significant(P>0.05).Conclusions The monitoring of HBV YMDD variation is important to the clinical therapy,prediction and prognosis.更多还原