【摘要】 目的鉴定大鲵皮肤分泌液中抗菌肽,研究其部分生物活性。方法 5%醋酸浸提和Sephadex G-50、G-25凝胶过滤色谱等方法分离纯化抗菌肽;采用抑菌圈法检测抗菌活性,Tricine-SDS-PAGE电泳和等电聚焦电泳鉴定其抗菌活性成份。结果大鲵皮肤分泌液中含有抗菌活性物质。对革兰阴性菌、革兰阳性菌和真菌均具有较强的抗菌活性;电泳检测显示该小分子多肽相对分子质量约为4 300,具有较强的碱性。结论首次从大鲵皮肤分泌液中分离纯化到一种抗菌肽,此抗菌肽可能是一个具有较强阳离子特征的碱性肽。更多还原

【Abstract】 Purpose To characterize the antimicrobial peptides from the skin secretions of the Andrias davidianus and to study its bioactivity.Methods This antimicrobial peptide was purified by 5%acetic acid extraction and Sephadex G-50,G-25 gel filtration chromatography.The antimicrobial ability was characterized by inhibiting zone determination.The antimicrobial active substance was identified by tricine-SDS-PAGE electrophoresis and isoelectric focusing(IEF) electrophoresis.Results The skin secretions of the Andrias davidianus contain antibiotic active substance.It strongly inhibited Gram-negative,Gram-positive bacteria and fungus.The electrophoresis results showed that its molecular weight was about 4 300 with a strong alkaline property.Conclusion An antibacterial peptide was isolated from the skin secretions of the Andrias davidianus for the first time and this antimicrobial peptide may be a strong feature of the basic peptide cation.更多还原