【摘要】 目的 观察人肺癌高转移细胞系SPC-A-1sci的转移相关基因表达，同时验证该细胞系的体内高转移特性。方法 通过Western blot、FICC、IHC等方法检测转移相关基因CD44,OPN,MMP-9,EGFR在高、低转移细胞系间的差异表达；采用尾静脉注射方法观察高、低转移细胞系的转移特性。结果 与低转移细胞系相比，转移促进基因CD44,OPN,MMP-9,EGFR在高转移细胞中的表达均显著上调。体内实验表明，8周时高转移细胞系的肺转移率为100%(12/12)，而低转移细胞系的肺转移率仅为16.67%(2/12)。结论 人肺癌高转移细胞系SPC-A-1sci在转移相关基因表达和动物体内均表现出典型的高转移特性，为该细胞系的推广应用提供了参考依据更多还原

【Abstract】 Objective To observe the metastasis-associated genes expression in the highly metastatic human lung cancer cell line SPC-A-1sci and validate the highly metastatic characteristics of SPC-A-1sci in vivo. Methods The expression levels of several metastasis promoting genes concluding CD44,OPN, MMP-9, EGFR were analyzed between the high and low metastatic cell lines by RT-PCR, Western blotting, FICC, IHC and so on. The metastatic characteristics was investigated by injection the cell lines into the tail vein of nude mice. Results Compared with the low metastatic cell lines, the metastasisassociated genes CD44, OPN, MMP-9, EGFR in highly metastatic cells were significantly increased.After 8 weeks in vivo experiments, 100%(12/12) of mice injected with highly metastatic cell lines developed lung metastases, while low metastatic cell lines was 16.67%(2/12). Conclusions The metastasisassociated genes are highly expressed in SPC-A-1sci, and the SPC-A-1sci shows the typical characteristics of highly metastatic in vivo, and may be served as a reference for the application of the cell lines.更多还原