【摘要】 观察鼠源性间充质干细胞株C3H10移植对实验性变态反应性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)模型小鼠疾病进程的影响,为研究多发性硬化症(multiple sclerosis,MS)等自身免疫性疾病的治疗提供实验基础。采用MOG35-55抗原与完全弗氏佐剂(IFA)免疫C57BL/6雌性小鼠制作EAE模型;24只小鼠分成三组,即未干预对照组、C3H10细胞移植组和正常对照组。通过HE染色,Fast-blue染色观察小鼠脑和脊髓的病理学改变,结合神经功能评分和体重测量的方法观察C3H10细胞移植对EAE小鼠的影响。结果:(1)动物行为学的改变:免疫后第10天开始,未干预对照组小鼠陆续出现EAE临床症状,第14至第18天达到发病高峰,平均神经功能评分为2.3±0.36(P<0.05),持续评分30d;C3H10细胞移植组小鼠有1只14 d和18 d神经功能评分为1分,正常对照组小鼠一直未出现临床症状(0分),持续观察30 d;(2)未干预对照组小鼠体重呈逐渐下降,第14天体重约(918.93±2.12)g,第22天约(18.65±2.04)g;C3H10细胞移植组小鼠体重有上升趋势,于造模后第14天称得体重为(18.7±0.93)g,造模后22 d小鼠体重保持在(19.26±0.58)g,两组相比具有统计学差异(P<0.05);(3)病理学改变:未干预对照组小鼠脑和脊髓小血管周围炎细胞浸润,呈袖套状或脱髓鞘改变,而C3H10细胞移植组和正常对照组小鼠未出现明显的脑和脊髓小血管炎症病理变化。小鼠间充质干细胞株C3H10移植于EAE小鼠可明显减少并延缓EAE发病,呈现一定的治疗作用。更多还原

【Abstract】 To observe the influence of transplantatiom of mesenchymal stem cells C3H10 on the disease process of experimental autoimmune encephalomyelitis(EAE) of mice to give the basis of experiments for research in the treatment of autoimmune disease,such as multiple sclerosis(MS),the EAE experimental model was established by immunization of female C57BL/6 mice with antigen MOG35-55 and complete Freund’s adjuvant(IFA),in which 24 mice were separated into three groups,i.e.non-intervene group,C3H10 transplantation group and normal group.Through hematoxylin-eosin and Luxol Fast-blue staining to observe the pathologic changes of mice and the neurological score with weight measures to observe effect of transplantation of mice with mesenchyma1 stem cells C3H10.(1) The changes of animal’s action:From 10 days after immunization,the non-intervention group emerged the clinical symbol,peaking in 14～18 day,and the average neurological score was about 2.3±0.36(P<0.05),while in the C3H10 transplantation group,the neurological score in two mice was about score,while normal group emerged no clinical symbol(0 score),which was kept for 30 days of continuous observation.(2) The weights of mice in non-intervention group kept decreasing by degrees,the actual weights measured about(18.93±2.12) g in 14 day,(18.65±2.04) g in 22 day,the weights of mice in the C3H10 transplantation group showed the tendency of increase up to(18.7±0.93) g and keeping the weight on(19.26±0.58) g after making model,indicating the statistical difference existing between them(P<0.05).(3) Pathological changes:Inflammatory cell infiltration was found around small blood vessels in the brain and spinal cord in non-intervention group,exhibiting cuffing or demyelinating changes,while on the C3H10 transplantation group and normal group,non of these clinical changes was emerged.It is evident that transplantation of mesenchymal stem cells C3H10 in EAE mice can decrease and delay the pathogenesis of mouse experimental autoimmune encephalomyelitis and thus shows some therapeutic effect as well.更多还原