【摘要】 目的筛选植物雌激素,并进一步研究植物雌激素对成纤维细胞合成胶原蛋白的影响。方法采用细胞增殖实验(MTT法)观察64种植物提取物或有效部位对雌激素受体(ER)阳性细胞系MCF-7增殖的影响,筛选具有雌激素样作用的植物提取物或有效部位;并用ER阴性的MDA-MB-231细胞和ER拮抗剂ICI 182780,考察促进MCF-7细胞的增殖作用是否由ER介导。采用圆点印迹法评估植物雌激素对成纤维细胞合成1型原胶原蛋白(PC-1)的影响。结果在筛选的64种植物提取物或有效部位中,葛根、决明子、啤酒花、桑叶、甘草植物提取物及大豆异黄酮可有效诱导MCF-7细胞增殖,但对MDA-MB-231细胞无诱导增殖作用;ICI 182780抑制其诱导MCF-7细胞增殖的作用。葛根素和染料木素显著促进人成纤维细胞合成PC-1(P<0.01),其中葛根素的作用较强;黄豆苷元则显著抑制PC-1合成(P<0.01)。结论葛根提取物和大豆异黄酮具有较强的雌激素样活性,主要的活性成分葛根素和染料木素可显著促进人成纤维细胞合成胶原蛋白,提示具有潜在的祛皱、抗皮肤老化的功效。更多还原

【Abstract】 Objective To screen phytoestrogens and investigate their effect on collagen synthesis in skin fibroblasts. MethodsSixty-four kinds of plant extracts were screened for phytoestrogens by determining their effect on estrogen receptor(ER)-positive MCR-7 cell proliferation with MTT assay.ER-negative MDA-MB-231 cells and ER antagonist ICI 182780 were used to validate if the stimulatory effect on MCF-7 cell proliferation was mediated by ER.By using dot blot method,the effect on procollagen-1(PC-1) synthesis in neonatal foreskin fibroblasts was also assessed. ResultsAmong the selected 64 plant extracts and active fractions,soybean isoflavonoids and the ethanol extracts of Radix Puerariae,Spica Humuli Lupuli,Semen Cassiae,Folium Mori and Radix Glycyrrhizae effectively induced the proliferation of MCF-7 cells,but showed no effect on MDA-MB-231 cells.Moreover,their effect on MCF-7 cell proliferation was inhibited by ICI 182780.Genistein and puerarin significantly increased PC-1 synthesis in skin fibroblasts(P<0.01),and puerarin showed more potent effect than genistein.In contrast,daidzein significantly reduced PC-1 synthesis(P<0.01). ConclusionThe extracts of Radix Puerariae and soybean isoflavnoids have relatively potent estrogenic activity.Puerarin and genistein,their main active ingredients,stimulate collagen synthesis of human skin fibroblasts,suggesting their potential anti-wrinkle and anti-skin aging effect.更多还原