【摘要】 目的探讨大肠癌组织中肿瘤相关巨噬细胞(TAMs)、转化生长因子β1(TGF-β1)表达与上皮间质转化(EMT)的关系。方法采用免疫组化法检测80例大肠癌及癌旁正常组织中CD68标记的TAMs、TGF-β1、上皮标志物E-cadherin、间质标志物Vimentin蛋白。结果大肠癌组织中TAM计数为(30.6±8.2)个/HP,TGF-β1蛋白阳性58例(72.5%),E-cadherin蛋白阳性31例(38.8%),Vimentin蛋白阳性38例(47.5%);而癌旁正常组织中分别为(16.4±7.2)个/HP、23例(28.8%)、64例(80.0%)、0例。两者比较,P均<0.01。TAMs计数及TGF-β1、E-cadherin、Vimentin蛋白表达与大肠癌分化程度、浸润深度、Duke分期和淋巴结转移有关(P均<0.05)。TAMs计数、TGF-β1与E-cadherin蛋白表达呈负相关(r值分别为-0.64、-0.39,P均<0.01),而与Vimentin蛋白表达成正相关(r值分别为0.62、0.33,P均<0.05)。结论大肠癌组织中TAMs浸润及TGF-β1高表达所造成的炎症环境可能与大肠癌的EMT有关。更多还原

【Abstract】 Objective To investigate the relationship between tumor-associated macrophages(TAMs),transforming growth factor β1(TGF-β1) expression and epithelial mesenchymal transition(EMT) in colorectal cancer tissue.Method Immunohistochemistry was used to detected the expression of TAMs marked CD68,TGF-β1,E-cadherin and Vimentin in 80 colorectal cancer specimens and matched adjacent non-cancerous tissue specimens.Result In colorectal cancer tissue,the TAMs count was 30.6 ±8.2 /HP,E-cadherin expression was positive in 31cases(38.8%),TGF-β1 expression was positive in 58 cases(72.5%),Vimentin expression was positive in 38 cases(47.5%).But 16.4±7.2 /HP,64 cases(80.0%),23 cases(28.8%),0 in adjacent non-cancerous tissue.TAMs count,TGF-β1,E-cadherin and Vimentin protein expression were correlated with differentiation,depth of invasion,Duke stages and lymph node metastasis in colorectal cancer,all P<0.05.TAMs count,TGF-β1 expression was negatively correlated with E-cadherin protein expression(r=-0.64 and-0.39,P<0.01) while positive correlated with Vimentin protein expression(r=0.62 and 0.33,P<0.05).Conclusion The infiltration of TAMs and TGF-β1 overexpressed caused inflammatory environment maybe involved in EMT in colorectal cancer.更多还原