【摘要】 目的探讨先天性心脏病(CHD)并发肺动脉高压(PH)患者肺组织中内皮型一氧化氮合酶(eNOS)与Ⅰ型和Ⅲ型胶原蛋白的表达情况及相互关系。方法光镜下观察60例CHD患者肺小动脉形态,按照Heath-EdwardsPH分级法分组:CHD无PH患者14例作为对照组,CHD合并PH患者46例为实验组,后者又分为Ⅰ、Ⅱ和Ⅲ级组。采用免疫组化SP法测定患者肺组织中eNOS和Ⅰ、Ⅲ型胶原蛋白的表达。结果与对照组比较,Ⅱ、Ⅲ级组Ⅰ、Ⅲ型胶原蛋白表达升高(P均<0.01)e,NOS蛋白表达降低(P<0.01)。Ⅰ、Ⅲ型胶原蛋白表达与PH分级呈正相关(P均<0.01),eNOS蛋白表达与PH分级呈负相关(P<0.01)。eNOS蛋白与Ⅰ、Ⅲ型胶原蛋白间存在明显负相关(P<0.05)。结论 eNOS可能参与CHD合并PH的肺血管重构过程。更多还原

【Abstract】 Objective To study the relationship between eNOS and collagenⅠ,Ⅲ expression in congenital heart disease(CHD) with pulmonary hypertension(PH).Methods Sixty patients with CHD were observed of pulmonary artery morphology and divided into 4 groups according to Heath-Edwards pulmonary vessel pathologic grading: control group(n=14),group Ⅰ(grade Ⅰ,n=18),group Ⅱ(grade Ⅱ,n=10),and group Ⅲ(grade Ⅲ,n=18).Immunohistochemical SP was employed to detect the expression of collagenⅠ,collagen Ⅲ and eNOS protein in the lung tissue of these patients.Results Compared with the control group,the expression levels of collagen Ⅰand Ⅲ were significantly increased in groupⅡ,Ⅲ,the expression levels of eNOS protein were significantly decreased in groupⅠ,Ⅱ,Ⅲ(P<0.01).There was a positive correlation between the expression level of collagen Ⅰ,Ⅲ and the grade of PH(P<0.01).There was a negative correlation between the expression levels of eNOS protein and the grade of PH(P<0.01).There was a negative correlation between the expression levels of eNOS protein and the collagen Ⅰand Ⅲ(P<0.05).Conclusion eNOS may play a role in the pathogenesis of pulmonary vascular remodeling.更多还原