【摘要】 目的观察IFN-γ诱导的供体脾脏树突状细胞(DC)对受体小鼠小肠移植排斥反应的影响,并探讨其机制。方法将供体、受体小鼠各36只分为A、B、C组各12对(供体、受体各12只),均制作小肠移植模型。A组造模前后不予任何处理;B、C组受体小鼠造模前5 d经尾静脉分别注射未诱导的和IFN-γ诱导的供体小鼠脾脏DC 2×106个。造模后,观察移植肠存活时间,HE染色观察移植肠排斥反应,采用RT-PCR、Western blot法检测肠组织中的吲哚胺2,3双加氧酶(IDO)mRNA及其蛋白。结果与A、B组比较,C组移植肠平均存活时间显著延长(P<0.01),排斥反应程度显著降低(P<0.05),IDO mRNA及其蛋白表达水平显著增高(P均<0.05)。结论 IFN-γ诱导的供体脾脏DC对受体小鼠小肠移植排斥反应有抑制作用,与其增加移植肠组织局部IDO表达有关。更多还原

【Abstract】 Objective To observe the effect of donor-derived DC induced by IFN-γ to acute rejection in mice SBT,and study its mechanism.Methods 36 donor mice and 36 recipient mice were divided into A,B and C groups(12 pairs each).Small bowel transplantation model was made all.Recipient mice in group A was treated with no treatments.Recipient mice in group B and C were separately injected with 2×106 donor-derived splenic DC which was not induced and induced by IFN-γ preoperatively.After operation,the survival of graft was observed.Grafts were individually collected for HE staining and pathological investigation;meanwhile,mRNA expression of IDO was assayed by RT-PCR,IDO protein was determined by Western blot.Results Comparing with group A and B,the graft survival of group C was significantly longer(P<0.01),the rejection level was downgrading(P<0.05).The IDO mRNA and protein expression levels were notably higher(P<0.05).Conclusions Preoperative medication with donor-derived DC induced by IFN-γ may inhibit rejection in mice SBT.This inhibition is relative with increasing IDO expression in graft.更多还原