【摘要】 目的探讨海南汉族乙型肝炎病毒(HBV)基因型与前C区G1896A和BCP区A1762T/G1764A基因突变的关系。方法采用RT-PCR方法检测乙型肝炎患者的HBV基因型,PCR方法扩增包含C启动子和前C区基因核苷酸(nt1643-nt2112),对PCR产物进行DNA测序。结果基因型C的BCP区A1762T/G1764A突变率(58.82%)显著地高于基因型B(10.53%)(P<0.05)。基因型C G1896A突变率为29.41%,基因型B G1896A突变率为47.37%,两者比较差异无统计学意义(P>0.05)。结论不同基因型HBV致病能力可能与病毒基因组BCP区A1762T/G1764A突变率的不同有关,而与前C区G1896A突变无关。更多还原

【Abstract】 Objective To investigate the relation between hepatitis B genotype and pre-core G1896A mutation and BCP A1762T/G1764A mutation in patients with chronic hepatitis B in Hainan.Methods HBV genotype was determined by real time fluorimetry polymerase chain reaction.Promoter C and ribonucleotide in pre-core C zone(nt1643-nt2112).DNA sequencing was used to PCR products.Results BCP A1762T/G1764A mutation frequency in patients infected with genotype C(58.82%) was higher than that of those with genotype B(10.53%)(P<0.05);G1896A mutation frequency was not significant difference between genotype C group(29.41%) and genotype B group(47.37%).Conclusions Pathogenecity of different HBV genotypes may relate to BCP A1762T/G1764A mutation frequency and have no relation with G1896A mutation.更多还原