【摘要】 目的探讨山茱萸环烯醚萜苷(cornel iridoid glycoside,CIG)对脑损伤大鼠海马区神经元存活的影响及其作用机制。方法 成年SD大鼠行穹隆海马伞切断(fimbria-fornix transection,FFT)手术,造模后CIG灌胃给药28d,采用尼氏染色方法光镜下观察海马CA1区和齿状回存活神经元的变化;采用Western blotting法检测海马区细胞凋亡调控因子Bcl-2、Bax和细胞色素C的蛋白表达变化。结果 尼氏染色结果显示,FFT模型大鼠海马CA1区和齿状回存活神经元明显减少;CIG(20、60、180mg.kg-1)灌胃给药能够增加模型大鼠海马区存活神经元的数量。Western blotting结果显示,FFT模型大鼠海马区Bcl-2表达减少,Bax和细胞色素C表达增高;CIG能够增强模型大鼠海马区Bcl-2表达,抑制Bax和细胞色素C的表达,避免凋亡信号进一步激活。结论 CIG能够减少FFT模型大鼠海马区神经元死亡数量,其作用机制可能与上调细胞凋亡抑制因子、下调细胞凋亡促进因子有关。更多还原

【Abstract】 Objective:To investigate the effects of cornel iridoid glycoside(CIG) on hippocampal neuron survival and its mechanism after brain injury in rats.Methods:Rat model of mechanical brain injury was induced by fimbria-fornix transection(FFT).CIG was intragastrically administered for 28 days after the operation.Nissl’s staining was used to evaluate neuron survival in hippocampal CA1 and dentate gyrus.Protein expression of apoptosis-regulating factors(including Bcl-2,Bax and cytochrome C) in the hippocampus of rats was detected by Western blotting.Results:Nissl staining showed a significant decrease in survived neurons in hippocampal CA1 and dentate gyrus of FFT model rats;the administration of CIG(20,60 and 180 mg·kg-1) significantly increased the number of neuronal survival,compared with the model rats.The protein expression of Bcl-2 was decreased,and the expression of Bax and cytochrome C was increased in hippocampus of FFT model rats;CIG significantly increased Bcl-2 expression and decreased the expression of Bax and cytochrome C,compared with the model rats.Conclusion:CIG markedly increased hippocampal neuronal survival in FFT model rats,and its mechanism may be related to the upregulation of apoptosis-inhibiting factors and downregulation of apoptosis-promoting factors.更多还原