【摘要】 目的研究肝硬化大鼠全肝血流阻断不同时间后肺组织损伤情况。方法 SD大鼠口服乙醇和CCl4制作肝硬化模型80只,随机分为4组,每组20只。假手术组(A1组)作为对照,采用阻断肝门、肝上上腔静脉及肝下下腔静脉15 min(A2组)、30 min(A3组)、45 min(A4组)制作全肝血流阻断模型,取右肺下叶肺组织常规HE染色病理检查,ELISA法检测肺匀浆TNF-α含量,左肺部分组织作湿/干比值(W/D)测定。结果①常规病理切片肺损伤评分:A4组评分最高,A2、A3组次之,A1组最低(P<0.05)。②ELISA法检测肺组织内TNF-α含量,A4组最高,A2、A3组次之,A1组最低(P<0.05)。③W/D:A4组最高,A2、A3组次之,A1组最低(P<0.001)。结论肝硬化大鼠全肝血流阻断后可以造成肺组织损伤,阻断时间超过30 min后明显加重。更多还原

【Abstract】 Objective To observe lung injury after total hepatic vascular occlusion in cirrhotic rats at different times.Methods A total of 80 SD rats developed liver cirrhosis by receiving alcohol and CCl4,and they were randomly divided into 4 groups(n= 20).The hepatic artery,portal vein,liver superior vena cava and hepatic inferior vena cava were blocked for 15 min(group A2),30 min(group A3) and 45 min(group A4) to establish a rat model of total hepatic vascular occlusion,rats free from occlusion(group A1) as controls.The lung tissue in the right lung lower lobe was taken for pathological examination by HE staining.The TNF-α level in lung homogenates was measured by ELISA.The wet/dry ratio was determined in part of the left lung lobe.Results The conventional pathological lung injury score ranked in a decreasing order from groups A4,A3,A2 to group A1(P<0.05),and the TNF-α level in the lung measured by ELISA and wet / dry weight ratio of the lung showed the same order(P<0.05 and P<0.001 respectively).Conclusion Lung injury can be caused after total hepatic blood flow occlusion in rats with liver cirrhosis,and the injury is significantly increased after occlusion for more than 30 min.更多还原