【摘要】 目的探讨天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)、骨桥蛋白(OPN)和埃兹蛋白(Ezrin)在乳腺浸润性导管癌(IDC)中的表达及其与临床病理因素的关系。方法应用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法检测71例术中切除的原发性乳腺IDC和33例距癌灶约5 cm处正常乳腺组织中Caspase-3、OPN和Ezrin的表达,分析3种蛋白间及其与乳腺IDC病理特征的关系。结果乳腺IDC组Caspase-3、OPN及Ezrin的阳性表达率分别为43.66%、74.65%、64.79%,正常乳腺组织对照组Caspase-3、OPN及Ezrin阳性表达率分别为75.76%、33.33%、39.39%,3指标在两组间表达差异均有统计学意义(P<0.05);在IDC组,3种蛋白的表达与乳腺IDC的淋巴结转移及肿瘤复发相关;Caspase-3、OPN及Ezrin蛋白的表达与雌激素受体(ER)、孕激素受体(PR)及原癌基因人类表皮生长因子受体2(Her-2)的表达无明显相关性;Caspase-3与OPN、Ezrin的表达呈负相关(r=-0.258、-0.313),OPN与Ezrin的表达呈正相关(r=0.29)。结论乳腺浸润性导管癌中Caspase-3、OPN、Ezrin 3种蛋白的异常表达在其发生、发展中可能起着重要的作用。更多还原

【Abstract】 Objective To investigate expressions of cysteinyl aspartate specific protease-3(Caspase-3),osteopontin(OPN) and Ezrin in invasive ductal carcinoma(IDC) of breasts and their relationship with clinicopathological characteristics.Methods Expressions of Caspase-3,OPN and Ezrin were detected in 71 IDC tissues and 33 normal mammary specimens with the immunohistochemical streptavidin-perosidase(SP) method,and their relationship with clinical pathological characteristics was analyzed.Results Positive rates of Caspase-3,OPN and Ezrin in IDC tissues(43.66%,74.65% and 64.79%,respectively) were significantly different from those in normal mammary tissues(75.76%,33.33% and 39.39%,respectively)(P<0.05).Also,expressions of Caspase-3,OPN and Ezrin in IDC tissues had a significant relationship with lymph node metastasis and recurrence of the tumor(P<0.05),while they had no significant relationship with expressions of ER,PR and Her-2.Expression of Caspase-3 was negatively related to those of OPN and Ezrin in IDC(r=-0.258,-0.313),while expression of OPN was positively related to that of Ezrin(r=0.29).Conclusion Abnormal expressions of Caspase-3,OPN and Ezrin play important roles in the occurrence and development of IDC of breasts.更多还原