【摘要】 目的研究β淀粉样蛋白(Aβ1-42)对胆碱能神经元ATP敏感钾离子(KATP)通道离子流的影响。方法原代培养出生24 h内的乳鼠皮层和海马胆碱能神经元,采用膜片钳全细胞记录技术记录Aβ1-42对单个胆碱能神经元KATP通道离子流的影响。结果与对照组比较,给予Aβ1-42后胆碱能神经元外向电流显著减少(P<0.05),再给予ATP敏感性钾通道开放剂二氮嗪后此减小的外向电流无显著变化(P>0.05)。结论 Aβ1-42对神经元KATP通道具有抑制作用。更多还原

【Abstract】 Objective To study the effect of β-amyloid peptides(Aβ1-42) on the current of ATP-sensitive potassium(KATP) channels in cultured cortical and hippocampal cholinergic neurons in rats.Methods Cortical and hippocampal cholinergic neurons from neonatal rats less than 24 h after birth were primarily cultured.Membrane currents were measured using patch clamp whole cell recordings.Results Compared with the controls,the outward current of neurons significantly decreased after using Aβ1-42(P<0.05).and there was no obvious change after giving diazoxide,an opener of KATP channels(P>0.05).Conclusion Aβ1-42 inhibits the opening of KATP channels.更多还原