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白介素-6对体外培养的黑素细胞影响的实验研究
Effects of interleukin-6 on cultured human melanocytes in vitro
【摘要】 目的建立正常人表皮黑素细胞体外培养体系,研究白介素-6(Interleukin-6I,L-6)对体外培养的黑素细胞的影响。方法采用四甲基偶氮唑盐(MTT)法测定白介素-6对黑素细胞活力的影响,NaOH裂解法测定黑素合成,流式细胞仪检测黑素细胞的凋亡率。结果白介素-6对黑素细胞活力有抑制作用,能使细胞增殖力降低,黑素合成减少,增加黑素细胞的凋亡率。结论白介素-6对体外培养的黑素细胞增殖及黑素生成都有抑制作用,这为临床应用白介素-6治疗色素性疾病提供了实验依据。
【Abstract】 Objective Establishing the culture of human normal melanocytes in vitro,and studying effects of Interleukin-6 on cultured human melanocytes.Methods MTT method,NaOH2 assay were employed to measure the cell viability,melanin synthesis of melanocytes,apoptosis rates of melanocytes were detected by using flow cytometer.Results Interleukin-6 induced a mild effect on melanocytic viability,while it showed a significant decrease in the cell counts,melanin synthesis and cell apoptosis.Conclusion Interleukin-6 can inhibit melanocytic proliferation and melanin synthesis.It is worth to study later.It makes valid to make use of Interleukin-6 for treatment of the pigmented skin diseases.
- 【文献出处】 四川医学 ,Sichuan Medical Journal , 编辑部邮箱 ,2011年03期
- 【分类号】R758.4
- 【被引频次】4
- 【下载频次】146