【摘要】 实验探讨了富硒酵母对斑点叉尾肝脏的毒性作用及蛋白质组学的影响,结果表明,饲养56 d后正常组和有机硒组采用原子荧光法检测硒含量均值为0.15和0.81 mg/kg。H.E染色显示有机硒组肝细胞有明显的疏松化,部分发生脂肪变。通过二维凝胶电泳结合ImageMaster软件分析,鉴定出8个表达差异蛋白质点,其中3个在有机硒组表达上调,5个在有机硒组特异表达,经液相色谱串联质谱分析鉴定,这些蛋白质分别是伴侣蛋白TCP1-亚基8、甘油醛-3-磷酸脱氢酶、4SNc-Tudor蛋白、腺苷激酶、酮糖移转酶、丙氨酰-tRNA合成酶。富硒酵母饲料对斑点叉尾肝具有明显的毒性效应,有机硒调控4SNc-Tudor蛋白,通过信号传导通道加强机体免疫。更多还原

【Abstract】 To investigate the influence on channel catfish(Ictalurus punctatus)liver toxicity and liver proteome change by the use of selenium-enriched yeast,Se-concentration in channel catfish liver was determined and the pathological change was monitored after selenium-enriched yeast feeding for 56 days.Proteins extracted from the liver of selenium-enriched yeast feeding channel catfishes and non-feeding controls were separated by two-dimensional gel electrophoresis(2-DE),differentially expressed proteins between the two groups were analyzed using ImageMaster software and identified by online reversed-phase nano-flow liquid chromatography coupled with ion trap mass spectrometry(LC-MS/MS).The average Se-concentration of liver form the non-feeding controls and the selenium-enriched yeast feeding ones were 0.15 mg/kg and 0.81 mg/kg.Significant difference was detected between these two groups(P<0.05).H.E staining of pathology from channel catfish liver showed that the selenium-enriched yeast group has obvious cell loose and cell fatty degeneration.15 differetial protein were found by 2-DE separation and ImageMaster software analysis.Of them,8 over-expressed proteins in selenium-enriched yeast were selected for MS analysis and identified successfully,including 3 u-regulated proteins and 5 uniquely expressed proteins in selenium-enriched yeast group compared with the controls.These differential proteins correspond to chaperonin TCP1 subunit 8 and glyceraldehyde-3-phosphate dehydrogenase,4SNc-Tudor domain protein,adenosine kinase a,transketolase and alanyl-tRNA synthetase-like et al.Obvious toxicity was detected in the channel catfish liver after feeding with selenium-enriched yeast.Organic selenium might strengthen immune through 4SNc-Tudor protein involved signal transmission pathway.Differential proteins identified in our work could be specific biomarkers related to toxicity of organic Se to channel catfish.更多还原