【摘要】 本文探讨了PKC的4种亚型α、βⅡ、γ和λ对P-TEFb各蛋白组分的表达影响.成功构建了带有HA标签的pcDNA3.1-PKC的各亚型真核表达载体,并转染HEK293细胞株,用Western bolt检测其表达情况,发现这四种PKC亚型在细胞中表达良好。将PKCβⅡ或者PKCλ转染进入HEK293细胞株,发现其可使CDK9和Cyclin T蛋白表达量升高;但在细胞中高表达PKCα和PKCγ,对P-TEFb各组分表达量没有影响.表明PKC可通过提高P-TEFb的组分表达量,从而上调某些基因的表达.更多还原

【Abstract】 The impact of PKC on the expression of P-TEFb was explored in this paper.PKCa、βⅡ、γandλwere ligated into the eukaryotic expression vector pcDNA3.1,and were transfected into the HEK293 cell line.The identity of PKCs was confirmed by anti-HA Western blot analysis.The PKC proteins were well expressed.When PKCβⅡor PKCλwas over-expressed,the expression level of CDK9 and Cylin T increased.PKCa and PKCλhad no impact on the level of P-TEFb.The conclusion was that PKC can increase the expression level of certain genes by increasing the amount of P-TEFb.更多还原