【摘要】 建立了奶粉中卵磷脂(PC)的高效液相色谱-蒸发光散射检测的测定方法,奶粉经去蛋白-液液萃取-富集浓缩超滤后进高效液相色谱仪,采用硅胶色谱柱,正己烷-异丙醇-水(体积比31∶62︰7)流动相体系,等度洗脱能够快速有效地分离奶粉中的卵磷脂。线性范围为0.020～1.60 g/L,相关系数为0.9996,检出限为15 mg/L,加标回收率为95.2%～96.8%。该方法用于实际样品的测定,结果令人满意。更多还原

【Abstract】 A method for the determination of milk-powder Phosphatidycholine(PC) by HPLC-ELSD was developed.PC in milk-powder was successfully separated on a SiO chromatography column,using hexane-isopropanol-water(31∶62∶7,v/v/v) as mobile phase after disposing protein,liquid-liquid extraction and concentration.The linear detection range was 0.020～1.60 g/L(r=0.9996) and the limit of detection(LOD) was 15 mg/L.The recovery was in the range of 95.2%～96.8%.The results of the actual sample indicated the method was accurate and reproducible.更多还原