【摘要】 以斑点叉尾鮰鱼排为实验材料,在一定条件下,利用中性蛋白酶和风味蛋白酶进行复合酶解。实验分析了六个单因素(温度、初始pH值、料液比、加酶量、中性蛋白酶和风味蛋白酶的复合比、水解时间)对酶解效果的影响,然后采用Box-Behnken中心组合设计和响应面分析,确定了最佳的工艺条件为:温度50℃,初始pH值7.0,料液比1∶2’酶的复合比1∶1,酶解时间5h,加酶量2 000U/g原料。此时,鮰鱼排水解度(DH)可达28.87%。更多还原

【Abstract】 The enzymatic hydrolysis of Channel catfish wastes(frames) with the complex of neutrase and flavourzyme was studied.The effects of several factors including hydrolysis time,solid-liquid ratio,quantity of enzyme,initial pH,hydrolysis temperature,the proportion of neutrase and flavourzyme were investigated.On the basis of single-factor experiments,the Box-Benhnken central composite design and response surface method were adopted to obtain the optimum conditions for enzymatic hydrolysis of Channel catfish wastes.The optimal enzymatic hydrolysis conditions were determined as:hydrolysis temperature 50℃,initial pH7.0,hydrolysis time 5.0 h,solid-liquid ratio 1:2,quantity of enzyme 2000 U/g and proportion of neutrase and flavourzyme 1:1.The degree of hydrolysis(DH) under optimum conditions could reach 28.87%.更多还原