【摘要】 【目的】通过观察中药复方脑力苏胶囊对血管性痴呆(VD)大鼠海马低氧诱导因子-1α(HIF-1α)阳性细胞表达的影响,探讨其改善VD大鼠认知障碍的作用机制。【方法】选用雄性SD大鼠90只,随机分为假手术组,模型组,脑力苏高、低剂量组(剂量分别为2.5、1.25 g.kg-1.d-1),喜得镇组(剂量为0.6 mg.kg-1.d-1);采用双侧颈总动脉结扎法复制VD大鼠模型,采用Morris水迷宫测试法检测VD大鼠学习、记忆改善状况,免疫组化法检测大鼠海马(CA1区)HIF-1α阳性细胞数。【结果】脑力苏高、低剂量组大鼠学习记忆能力显著改善(P<0.01),海马HIF-1α阳性细胞表达较模型组显著降低(P<0.01)。【结论】脑力苏胶囊治疗VD的作用与其能降低海马HIF-1α表达,提高缺血脑组织细胞内氧浓度有关。更多还原

【Abstract】 Objective To investigate the influence of Naolisu Capsules(NC) on hypoxia inducible factor alpha(HIF-α) expression in the hippocampus of rats with vascular dementia(VD) induced by chronic ischemia,and to explore the mechanism of NC on improving cognitive disorder in VD rats.Methods SD rats were randomized into sham-operation group,model group,dihydroergotoxine group(in the dosage of 0.6 mg·kg-1·d-1),and high-and low-dosage NC groups(in the dosage of 2.50 and 1.25 g·kg-1·d-1,respectively).VD rats models were established by ligation of bilateral common carotid artery.Morris water maze was used for the examination of learning and memory of VD rats.Immunohistochemical method was applied for the detection of the number of cells with positive expression of HIF-α.Results Learning and memory were obviously improved,and the number of cells with positive expression of HIF-α in the hippocampus was decreased in NC groups(P<0.01 compared with those in the model group).Conclusion The therapeutic mechanism of NC for VD is related with the decrease of hippocampal HIF-α expression and with the increase of cellular oxygen concentration in ischemic brain tissue.更多还原