【摘要】 目的:观察参芪化瘀方对荷瘤小鼠肿瘤组织p53基因表达的影响,探讨参芪化瘀方对H22肝癌细胞作用的机制。方法:将H22肝癌荷瘤小鼠60只随机分为6组各10只:参芪化瘀方高剂量组、中剂量组、低剂量组,环磷酰胺注射用粉针剂(CTX)对照组,模型组,阴性对照组。参芪化瘀方高、中、低剂量组予以0.4mL中药(浓度分别为4.16g/mL、2.08g/mL、1.04g/mL)灌胃,CTX对照组予以0.2mL[小鼠给药剂量为20mg/(kg.d)]腹腔注射,模型组予以0.4mL生理盐水灌胃,每天1次,给药14天。免疫组织化学法检测肿瘤组织p53基因的表达,并观察其对脾脏指数、胸腺指数及肿瘤抑制率。结果:高、中、低剂量参芪化瘀方都能降低H22肝癌荷瘤小鼠肿瘤组织中p53基因的表达水平,且能升高小鼠的脾脏指数、胸腺指数。结论:参芪化瘀方对H22肝癌荷瘤小鼠肿瘤组织中p53基因的表达具有一定抑制作用,且能保护小鼠的免疫器官。更多还原

【Abstract】 Objective:To observe the effect of Shenqi Huayu Formula on p53 gene expression in tumor-bearing mice,and to explore its mechanism in influencing H22 hepatoma cells.Methods:Sixty mice transplanted with H22 hepatoma cells were randomly divided into six groups:high-,middle-and low-dose Shenqi Huayu Formula groups(gastric gavage of 0.4mL of the decoction of Shenqi Huayu Formula),cyclophosphamide(CTX) group [intraperitoneal injection of 0.2mL of CTX at the concentration of 20mg/(kg·d)],model group(gastric gavage of 0.4mL of normal saline),and negative control group.The medication was carried out once a day and lasted 14 days.The expression of p53 gene in the tumor tissue was tested by immunohistochemical assay.Spleen index,thymus index and tumor-inhibition rate were also observed.Results:The expression of p53 gene in all of Shenqi Huayu Formula groups was reduced,and the spleen index and thymus index were increased.Conclusion:Shenqi Huayu Formula can inhibit the expression of p53 gene and protect the immune organs of H22 tumor-bearing mice.更多还原