【摘要】 目的研究含人IL-21基因的重组腺病毒(Ad-IL-21)联合γ射线照射对子宫颈癌Hela细胞生长的抑制作用。方法重组腺病毒Ad-IL-21于体外转染子宫颈癌Hela细胞,转染后6 h进行6Gy 137Csγ射线照射,用MTT方法和流式细胞仪测定Hela细胞的生长曲线、抑制率及细胞周期。结果 Ad-IL-21基因组、照射组和基因联合照射组对Hela细胞生长均具有抑制作用,联合照射组的抑制效应最强(44%),明显高于Ad-IL-21组和照射组。联合照射组Hela细胞出现明显的G1期阻滞,G1期细胞所占比例最高,达到88.9%,S期细胞最少(1%)。结论 IL-21基因联合γ射线照射对子宫颈癌的抑瘤作用具有协同效应,有效地抑制肿瘤细胞的生长。更多还原

【Abstract】 Objective To study the effect of inhibition on the growth of cervical carcinoma cells by introduction of foreign IL-21 gene vector with γ-ray.Methods The recombinant IL-21 gene adenovirus vector was transfected into cervical carcinoma cell line Hela.Hela cells were irradiated with 6Gy 137Cs γ-ray after 6 hours transfection The cells growth curve and cells cycle of Hela cells were detected by MTT assay and flow cytometry respectively.Results The growth of Hela cells transfected with Ad-IL-21,radiation and Ad-IL-21 combined with radiation group was all inhibited.The group of Ad-IL-21 combined with radiation resulted in greater inhibition of cells growth,reaching 44%,compared with Ad-IL-21 infection and radiation group.Cells of G1 phases of Hela cells for Ad-IL-21 combined with radiation group were the highest,reaching 88.9%,while those of S phases were the lowest,reaching 1%.Conclusion Ad-IL-21 gene transfer combined with radiation show synergism for the inhibition of cervical cancer Hela cells growth.更多还原