Objective To assess the value of double PCR in clinical diagnosis of suspected three-fever malaria cases by comparison with conventional microscopy in malaria endemic areas.Mthods The peripheral blood samples of 334 suspected three fever malaria outpatients were collected and chelex-100 DNA template were made and detected with double PCR and at the same time compared with microscopy of thick smear for malaria parasites.Results The sensitivity of double PCR in diagnosios of malaria was 96.1%,and a missing ex...