【摘要】 目的:探讨银杏叶提取物(extract761 from Ginkgo giloba,EGb761)对黄曲霉毒素B1(aflatox B1,AFB1)致大鼠肝癌后相关蛋白表达的影响。方法:71只大鼠随机分为AFB1组(28只),AFB1+EGb761组(29只),空白对照组(14只)。第64周观察大鼠肝癌发生率,应用免疫组化法检测硫酸乙酰肝素类蛋白聚糖(MXR7)、环氧化酶(COX-2)以及细胞周期蛋白依赖性蛋白激酶抑制蛋白(p16)的表达情况。结果:AFB1组肝癌发生率明显高于AFB1+EGb761组(76%vs28%),χ2=10.602,P<0.05,对照组无肿瘤发生。AFB1+EGb761组MXR7蛋白含量明显低于AFB1组〔(1.488±1.178)vs(5.133±2.725)〕,F=18.638,P<0.001;AFB1+EGb761组p16蛋白含量明显高于AFB1组〔(2.456±1.014)vs(1.533±0.856)〕,F=24.091,P=0.03,AFB1组COX-2蛋白含量为3.305±0.566;AFB1+EGb761组为2.704±0.431,F=9.352,P=0.783。结论:银杏叶提取物下调大鼠肝组织MXR7蛋白的表达,提高p16蛋白的表达,有抑制AFB1致大鼠肝癌的发生作用。更多还原

【Abstract】 OBJECTIVE: To investigate the effects of Ginkgo biloba extract(extract 761 from Ginkgo giloba,EGb761) on relative protein expressions during hepatocarcinogenesis induced by aflatoxin B1(AFB1) in rats.METHODS: The 71 rats were divided into three groups: Group AFB1(28),Group AFB1+EGb761(29) and Group control(14).The animals were sacrificed at the end of the experiment.The incidence of liver cancers in each group was stated.The expressions of heparan sulfate proteoglycan(MXR7),Cycloxygenase-2(COX-2) and Cyclin dependent kinase inhibitor(P16) were observed by immunohistochemistry staining in above samples.RESULTS: The rate of hepatocellular carcinoma(HCC) in group AFB1 were significantly higher than group AFB1+EGb761(76% vs 28%,χ2=10.602,P<0.05).No tumor developed in the control animals.The expression of MXR7 protein in group AFB1 + EGb761 were significantly lower than group AFB1(1.488±1.178) vs(5.133±2.725),(F=18.638,P<0.001).p16 protein in group AFB1+EGb761 were significantly higher than group AFB1(2.456±1.014) vs(1.533±0.856),(F=24.091,P=0.03).The difference of COX-2 protein expression in group AFB1 and group AFB1 + EGb761 had no significant(3.305±0.566) vs(2.704±0.431),(F=9.352,P=0.783).CONCLUSION: Ginkgo biloba extract reduces MXR7 protein expression in rats and enhance the expression of p16 protein,and it may be inhibited the occurrence of liver cancer induced by AFB1.更多还原