【摘要】 目的:观察不同浓度的吉非替尼(gefitinib)联合不同剂量放疗对人肝癌Bel7402细胞株的放射增敏作用。方法:采用MTT法测定浓度分别为0、1、2.5、5、10、20、40和80μmol/L Gefitinib对肝癌Bel7402细胞株的生长抑制率;克隆形成实验测定SF(存活分数),观察放射敏感性,计算细胞生存率,拟合生存曲线并计算放射增敏比,观察放射增敏作用。结果:MTT实验结果表明,Gefitinib单独作用于肝癌Bel7402细胞株具有对细胞生长抑制作用,其IC50(半数抑制浓度)为7.26μmol/L。不同浓度的Gefitinib与不同剂量照射人肝癌Bel7402细胞株,SF与单纯放射治疗相比其差异均有统计学意义,P<0.05。多靶单击模型,拟合生存曲线为人肝癌Bel7402细胞株R(放疗)+0.1×IC50Gefitinib、R+0.2×IC50Gefitinib生存分数其放射增敏比(SER)分别为1.237 5和1.345 6。结论:Ge-fitinib对肝癌Bel7402细胞株有抑制增殖作用,其IC50值为7.26μmol/L。Ge-fitinib对肝癌Bel7402细胞株有放射增敏作用,并随着药物浓度的增加,通过放射生物学参数及细胞生存曲线表明其放射增敏作用增强。更多还原

【Abstract】 OBJECTIVE: To explore whether different concentration of Gefitinib combinated with diffenent dose of radiotherapy enhance the radiosensitization to human hepatocellular carcinoma Bel7402 cell line in vitro.METHODS: The inhibitory effect of different concentration of Gefitinib on cell proliferation was measured by MTT assay,which concentration was 0,1,2.5,5,10,20,40,80 μmol/L,and learn the value of IC₅0 and the degree of inhibition by different concentration of drug.Clonogenic assay:to observe the radiosensitization enhancement induced by gefitinib by observing the number of cell cloning calculation of cell survival and to calculate cell survival curve and the calculate radiobiology parameter.RESULTS: MTT experimental results showed that Gefitinib used alone had significantly inhibited effect on the human hepatocellular carcinoma Bel7402 cell line in vitro.The 50% inhibition concentrations（IC₅0） of Gefitinib were 7.26 μmol/L for Bel7402 cells.To SF of the Bel7402 cell which were treated by different concentration of Gefitinib with different dose of radiotherapy compared to the radiation treatment group,there was significant difference（P<0.05）.Applying multitarget single-hit model,observing the Bel7402 cell survival in different condition of R+0.1×IC₅0 Gefitinib and R+0.2×IC₅0 Gefitinib,fit cell survival curve,in order to calculate the rate of SER seperately which was 1.237 5 and 1.345 6.CONCLUSIONS: Gefitinib can significantly inhibit the proliferation of Bel7402 cells.IC₅0 is 7.26 μmol/L,proliferation inhibition rate increased along with increased concentrion of Gefitinib.The radiosensitivity effcet of Bel7402 cells can be enhanced by Gefitinib concentrations in the low cytotoxicity.The effect of radiosensitization increase gradually with the increment of concentrion.Survival curves and cell biololgical parameters of radiation show the enhancing of their radiosensitivity.更多还原