【摘要】 目的:评价DNA定量细胞学配合液基细胞学检查在宫颈癌防治中的价值。方法:收集2009-01-01-2010-10-31在我院妇科门诊行液基细胞学及DNA定量检查的患者4 352例,对检查结果建议为活检的病例504例行阴道镜检查及活体组织检查,常规HE染色后观察病变程度,分别计算其与液基细胞学和DNA Feulgen染色后的阳性率。结果:以TBS活检标准行活检病例的阳性检出率为52.18%(263/504),以DNA定量分析结果活检标准行活检的病例阳性检出率为66.67%(336/504),经2种细胞学方法联合建议取活检后,其阳性检出率为81.75%(412/504),3种方法两两比较,差异有统计学意义,P<0.05。结论:DNA Feulgen染色和液基细胞学联合检测既能降低宫颈液基细胞学的假阴性率,又能提高宫颈早期病变的检出率,为防止宫颈早期病变进一步发展为癌起到一定的积极作用。更多还原

【Abstract】 OBJECTIVE: To evaluate the value of the DNA quantitative cytology with liquid-based cytology in cervical cancer prevention and treatment.METHODS: 4 352 cases of patients in our hospital outpatient gynecologic from 01-01-2009 to 10-31-2010 were involved in this study.All the 504 cases which were recommended biopsy were conducted colposcopy and biopsy.The pathological changes were observed after the routine HE staining.Then the positive coincidence rate of the liquid-based cytology was calculated with routine HE staining and DNA Feulgen staining with routine HE staining respectively.RESULTS: The positive rate was 52.18%(263/504)with the diagnosis of the TBS biopsy criteria.The positive rate was 66.67%(336/504) with the diagnosis of the DNA Feulgen staining biopsy criteria,While the positive rate was 81.75%(412/504) with the diagnosis of the two combined cytological method biopsy criteria.There was significant difference among the three methods(P<0.05).CONCLUSIONS: DNA Feulgen staining compared with the single liquid-based cytology,the rate of early detection of cervical lesions is improved significantly.The false negative rate of the liquid-based cytology can be reduced by the combination of the two,but also the detection rate of cervical lesions of early can be improved.It plays a posive role in order to prevent further development of lesions of early cervical cancer.更多还原