【摘要】 目的:分析Axin和DNA甲基转移酶1(DNMT1)在肺癌组织中的表达和相关性,及其与肺鳞癌和腺癌临床病理因素的关系。方法:采用免疫组化方法检测Axin和DNMT1在100例肺癌组织中的表达情况。结果:Axin在肺鳞癌和腺癌组织中表达明显减少,其细胞质表达与肺癌的分化程度相关(P=0.044),核表达与肺癌的组织学类型(P=0.001)和TNM分期(P=0.016)相关。DNMT1在肺癌组织中的表达明显增加,并与肺鳞癌和腺癌的组织学类型(P=0.005)、分化程度(P=0.038)、TNM分期(P=0.031)和淋巴结转移(P=0.032)显著相关。Axin在肺癌组织中的低表达与DNMT1高表达显著负相关(r=-0.222,P=0.027),而在肺腺癌组织中Axin的核表达也与DN-MT1的表达显著负相关,r=-0.308,P=0.026。结论:Axin在肺鳞癌和腺癌组织中的低表达与DNMT1的高表达显著相关。DNMT1的表达可以作为肺癌恶性程度和进展情况的指标之一。更多还原

【Abstract】 OBJECTIVE: To examine the expressions and correlations of Axin and DNA methyltransferase 1(DNMT1) in lung cancers,and their relationships with the clinicopathological parameters of lung squanous cell carcinomas and adenocarcinomas.METHODS: The expression patterns and levels of Axin and DNMT1 were examined using immunohistochemistry in 100 lung cancer tissue samples.RESULTS: The expression of Axin was reduced significantly in lung cancers.The cytoplasmic expression of Axin was associated with the differentiation(P=0.044) of lung cancers;the nuclear expression of Axin was associated with the histological type(P=0.001) and TNM stage(P=0.016) of lung cancers.The expression of DNMT1 was enhanced significantly,and associated with the histological type(P=0.005),differentiation(P=0.038),TNM stage(P=0.031) and lymph node metastasis(P=0.032) of lung cancer samples.The reduced expression of Axin was significantly correlated with the high expression of DNMT1(r=-0.222,P=0.027).Furthermore,the nuclear expression of Axin was also negatively correlated with the expression of DNMT1 in lung adenocarcinoma tissues(r=-0.308,P=0.026).CONCLUSIONS: The reduced expression of Axin is correlated with the high expression of DNMT1 in lung cancers.The expression level of DNMT1 can be a mark to indicate the grade malignancy and progression of lung cancers.更多还原