【摘要】 目的:探讨健康人和胃癌患者细胞因子诱导的杀伤细胞(CIK)对原代癌细胞的体外抗肿瘤作用的差异,进一步了解CIK细胞的抗肿瘤作用。方法:分离健康人和胃癌患者自身的外周血单个核细胞,分别加入鼠抗人CD3 McAb、基因重组人IL-1α、IFN-γ、IL-2,经体外培养诱导为CIK细胞;手术留取的新鲜胃癌组织用机械研磨法分离成单细胞悬液;用四甲基偶氮唑盐(MTT)法检测2种CIK细胞对胃癌细胞的杀伤活性。结果:健康人的CIK细胞较胃癌患者自身的CIK细胞的体外增殖力强,第1天表达CD3+/CD56+的细胞健康人占(1.064±0.22)%,癌患者占(1.031±0.13)%;而经细胞因子刺激后的第7天健康人占(28.66±2.00)%,癌患者占(17.63±2.22)%;14 d后分别占(57.14±1.96)%和(48.53±2.19)%(P<0.05);其杀伤肿瘤的能力也远远高于患者自身的杀伤能力。结论:健康人和胃癌患者的CIK细胞对原代癌细胞都有细胞毒的作用,健康人的CIK细胞无论增殖能力和细胞毒的活性都远高于肿瘤患者自身的CIK细胞。更多还原

【Abstract】 OBJECTIVE: To explore the anti-tumor function differences of original generation CIK cells between normal and stomach neoplasms patients,and detect CIK cells anti-tumor effect.METHODS: Individual nucleus cells of normal people and gastric cancer patients were separated,and CD3McAb,IL-1α,IFN-γ,IL-2 were added respectively.MTT method was used to detect kill activity of two CIK cells.RESULTS: On the first day,CD3+/CD56+ was expressed in(1.064±0.22)% normal people and(1.031±0.13)% gastric cancer patients.After the cell factors stimulation,the expression rates were(28.66±2.0)%,and(17.63±2.22)% on the 7th day,and(57.14±1.96)% and(48.53±2.19)% on the 14th day accounted for(P<0.05).Its kill tumor ability also was higher than that of the patient’s own killer ability(P<0.01).CONCLUSION: Both the normal people and gastric cancer patients CIK cells have anti-tumor effect,and the normal people’s CIK cell proliferation capacity and anti-tumor activity are higher than those of the tumor patients.更多还原