【摘要】 目的探讨蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系环氧合酶-2(COX-2)表达的影响。方法用不同浓度的Agkihpin处理鼻咽癌CNE-2细胞系72 h后,应用免疫细胞化学、Western Blotting、反转录-聚合酶链反应(RT-PCR)法检测COX-2在CNE-2细胞系中的表达。结果不同浓度Agkihpin处理CNE-2细胞系72h后,COX-2的平均吸光密度比较,差异有统计学意义(P<0.05);其中A1组、B1组和C1组较D1组均明显降低,差异有统计学意义(P<0.05),A1组较B1组、C1组和D1组均明显降低,差异有统计学意义(P<0.05)。WesternBlotting检测经不同浓度Agkihpin处理72 h后COX-2表达差异有统计学意义(P<0.05),随着Agkihpin浓度的降低,COX-2表达水平升高,各组间比较差异均有统计学意义(P<0.05)。不同浓度Agkihpin处理72 h后COX-2 mRNA的相对转录水平比较差异有统计学意义(P<0.05),随着Agkihpin浓度的降低,COX-2 mRNA的相对转录水平升高,各组间比较差异均有统计学意义(P<0.05)。结论在人低分化鼻咽癌CNE-2细胞系中,Agkihpin能抑制COX-2的表达,并且随着浓度的增大更明显;Agkihpin对COX-2的抑制作用,可能成为鼻咽癌化疗的有效药物。更多还原

【Abstract】 Objective To explore the impact of Agkihpin on the expression of cyclooxygenase-2 in nasopharyngeal carcinoma CNE-2 cell line and to describe the mechanisms of Agkihpin inhibition against CNE-2.Methods The cultured cells were treated with different concentrations of Agkihpin for 72 h.The expression levels of COX-2 in CNE-2 cell line were assayed by immunocytochemistry,Western blotting,and RT-PCR.Results The average COX-2 absorbance among different Agkihpin concentrations showed significant differences after 72 hours of treatment(P<0.05);more specificly,the measurement was lower in group A,B,C than in group D(P<0.05) and lower in group A than in group B,C,D(P<0.05).Western Blotting showed significant differences among different Agkihpin concentrations after 72 hours of treatment(P<0.05);COX-2 expression increased as Agkihpin concentration decreased(P<0.05).Expression levels of COX-2 mRNA showed significant differences among different Agkihpin concentrations after 72 hours of treatment(P<0.05);COX-2 expression increased as Agkihpin concentration decreased(P<0.05).Conclusion Agkihpin inhibits the expression of COX-2 in CNE-2 cells in a dose-dependent manner,which is likely one of the reasons for Agkihpin inhibition of the cellular vitality of nasopharyngeal carcinoma cells;Agkihpin might be applied in the treatment of nasopharyngeal carcinoma.更多还原