【摘要】 目的研究结直肠癌患者门静脉血、外周血中p27基因甲基化状态的变化,探讨其与临床病理特征的关系。方法应用甲基化特异性聚合酶链反应分析技术,检测106例结直肠癌患者的门静脉血中p27基因启动子甲基化的阳性率,并结合临床病理进行分析。结果结直肠癌患者外周血中的p27基因甲基化阳性率为24.5%(26/106),对照组为3.4%(1/29)。结直肠癌患者门静脉血中p27基因甲基化阳性率为30.2%(32/106),其中Dukes分期:A+B期和C+D期阳性率分别为20.0%(11/55)和41.2%(21/51);低分化和高中分化阳性率分别为48.0%(12/25)和24.7%(20/81);有无淋巴结转移阳性率分别为41.5%(17/41)和23.1%(15/65);浸润深度:未达浆膜层和达浆膜层阳性率分别为24.1%(7/29)和32.5%(25/77)。门静脉血中p27基因甲基化与结直肠癌患者的临床分期、组织分化程度及有无淋巴结转移均有关(P<0.05),但与患者的性别、年龄、肿瘤部位和大小均无统计学意义(P>0.05)。结论结直肠癌的发生发展与p27基因甲基化有关。检测结直肠癌患者门静脉血中p27基因甲基化程度,可为临床分期及预后的判断提供依据。更多还原

【Abstract】 Objective To investigate the methylation status of p27 gene promoter in portal vein blood and peripheral blood of colorectal carcinoma patients and its relationship with the clinical characteristics of the disease.Methods The methylation status of p27 gene promoter in portal vein blood in 106 patients with colorectal carcinoma was detected using methylation-specific PCR(MSP).Blood samples from 29 age-matched healthy subjects were adopted as controls.Results Positive rate of p27 gene methylation in the peripheral blood of colorectal carcinoma patients were merely 24.5%(26/106),compared to 3.4%(1/29) in the control subjects,while in portal vein blood the positive rate went up to 30.2%(32/106).Positive rates were 20.0%(11/55) and 41.2%(21/51) in patients of Duke A+B and Duke C+D,48.0%(12/25) and 24.7%(20/81) in patients with low and middle-high differentiation,41.5%(17/41) and 23.1%(15/65) in patients with and without lymph node metastasis,and 24.1%(7/29) and 32.5%(25/77) in patients with and without serosa invasion,respectively.Hypermethylation status in p27 promoter was significantly correlated to staging,degree of differentiation,and status of lymph node metastasis(P<0.05) and it was not significantly correlated to age,sex,neoplasm position and size(P>0.05).Conclusion Hypermethylation in p27 gene promoter is associated with pathogenesis and development of colorectal carcinoma,which in turn might provide evidences for diagnosis and prognostic prediction of the disease.更多还原