【摘要】 目的探讨环氧合酶-2(COX-2)在胃癌淋巴结转移灶中的表达及其与生存素(Survivin)、细胞核增殖抗原(Ki-67)表达的关系。方法对40例伴淋巴结转移的胃癌手术标本的原发灶、淋巴结转移灶进行COX-2、Survivin、Ki-67免疫组化染色,比较三者在淋巴结转移灶及原发灶中表达的差异。结果 (1)COX-2、Survivin在原发灶中强表达率分别为52.5%(21/40)、70.0%(28/40);在淋巴结转移灶中分别为72.5%(29/40)、72.5%(29/40)。COX-2在淋巴结转移灶中的表达显著高于原发灶,差异有统计学意义(χ2=4,P<0.05);但Survivin在淋巴结转移灶与原发灶中的表达强度比较差异无统计学意义(χ2=0.0667,P>0.05)。原发灶中Ki-67指数显著高于淋巴结转移灶(t=-2.17,P<0.05)。(2)双变量相关分析显示,原发灶中COX-2、Ki-67的表达与淋巴结转移灶中二者的表达均呈正相关(P<0.05)。(3)在原发灶中,COX-2与Survivin、Ki-67的表达均呈正相关(r值分别为0.6954和0.6084,P<0.01),Survivin与Ki-67的表达也呈正相关(r=0.6402,P<0.01);在淋巴结转移灶中,COX-2与Ki-67的表达呈正相关(r=0.3350,P<0.05)。结论胃癌淋巴结转移灶中COX-2表达与原发灶比较已经出现异质性变化;COX-2可能通过调控Survivin、Ki-67的表达而参与胃癌细胞的增殖和凋亡过程。更多还原

【Abstract】 Objective To investigate the expression of COX-2 in lymph node metastases(LNMs)of gastric carcinomas and its relationship to Survivin and Ki-67.Methods The expressions of COX-2,Survivin and Ki-67 were determined immunohistochemically in 40 paired primary tumors(PT) and LNMs of gastric carcinomas.Results The strong positive expression rates of COX-2 and Survivin were 52.5%,70.0%,respectively,in PT,and 72.5%,72.5%,respectively,in LNMs.The expression of COX-2 was higher in LNMs than in PT,the difference was significant(χ2=4,P<0.05).But no significant difference was noted in expression of Survivin between in PT and in LNMs(χ2=0.0667,P>0.05).The Ki-67 labeling index was higher in PT than in LNMs(t=-2.17,P<0.05).The expressions of COX-2 and Ki-67 in PT were positively correlated with those in LNMs(r=0.3484,0.5817,both P<0.05),COX-2 positively with Survivin and Ki-67(r=0.6954,0.6084,both P<0.01),and Survivin positively with Ki-67(r=0.6402,P<0.01);in LNMs,COX-2 positively with Ki-67(r=0.3350,P<0.05).Conclusion COX-2 expression in LNMs shows heterogeneity changes as compared with in PT.COX-2 may participate in cancer cell proliferation and apoptosis by regulating expressions of Survivin and Ki-67.更多还原