【摘要】 目的探讨β-榄香烯联合顺铂和热疗对A549细胞中HSP70、MDR1、Bax/Bcl-2基因表达的影响。方法 15μg/ml和60μg/ml榄香烯分别联合热疗和顺铂处理细胞后,RT-PCR及WesternBlot法检测HSP70-1、MDR1基因mRNA转录和蛋白表达;RT-PCR检测Bax/Bcl-2基因mRNA转录水平。结果各实验组HSP70-1基因mRNA转录水平差异无统计学意义,而各实验组HSP70蛋白表达水平除了37℃下顺铂4μg/ml组、榄香烯15μg/ml、顺铂4μg/ml联合60μg/ml榄香烯组外其余各组均显著升高,差异有统计学意义(t分别=7.48～55.76,P均<0.05);37℃下各实验组MDR1基因mRNA转录多数降低,顺铂4μ/ml联合榄香烯15μg/ml与37℃对照组相比,差异有统计学意义(t=93.38,P<0.05),42℃下顺铂4μg/ml联合15μg/ml榄香烯组与42℃对照组比较,差异具有统计学意义(t=7.99,P<0.05)。各实验组MDR1蛋白表达均较低,与对照组比较,差异无统计学意义;部分实验组Bcl-2基因mRNA水平降低,与对照组比较,差异具有统计学意义(t分别=4.41、3.97、5.11、3.43、4.88、4.52,P均<0.05),而Bax基因mRNA水平未见明显变化。结论 37℃下高浓度榄香烯联合顺铂可能是通过抑制MDR1、Bcl-2等耐药相关基因协同杀伤细胞,而42℃下低浓度榄香烯和顺铂主要是通过抑制Bcl-2基因活性起到协同杀伤效应。更多还原

【Abstract】 Objective To investigate the effect on expression of HSP70,MDR1 and Bax/Bcl-2 in A549 cells treated by β-elemene with hyperthermia and cisplatin.Methods Treated by β-elemene,hyperthermia and cisplatin,RT-PCR and Western Blots were applied to detect mRNA transcription and protein expression of HSP70 and MDR1,while Bax/Bcl-2 mRNA detected by RT-PCR.Results No significant difference was observed in HSP70 mRNA transcription,but compared with control at 37℃.HSP70 protein expressions were mostly up-regulated and there was statistical significance(t=7.48～55.76,P<0.05).MDR1 mRNA were mostly down-regulated,but after treatment with cisplatin 4 μg/ml and 15 μg/ml elemene in 42℃ it was up-regulated(compared with 37℃ control,or 4 μg/ml cispaltin and 15 μg/ml elemene in 42℃,t=93.38,7.99,P<0.05).Bcl-2 mRNA transcriptions were mostly down-regulated and significant differences were observed(t=4.41,3.97,5.11,3.43,4.88,4.52,P<0.05)compared with control,while Bax mRNA with no significant differences.Conclusions Inhibition of MDR1 and Bcl-2 result cell apoptosis when treated after high concentration elemene and cisplatin at 37℃,but after treated with low concentration elemene and cisplatin at 42℃ the cell apoptosis mainly owed to the lower expression of Bcl-2 gene.更多还原