【摘要】 单环刺螠纤溶酶(UFE)是由本实验室分离纯化出的1组包括4个分子量组分的纤溶酶,各个组分均有提高受试机体继发性纤溶活性的能力,并具有显著的抗凝活性和纤溶活性以及较好的生物安全性。根据该纤溶酶Ⅱ的N-末端氨基酸序列设计简并引物,通过3-’RACE PCR获得该组分蛋白质的部分编码序列,进一步通过5-’RACE PCR获得单环刺螠纤溶酶基因全长cDNA编码序列906 bp,其中开放阅读框795 bp。该基因全长cDNA BLASTx比对结果表明,其编码产物与丝氨酸蛋白酶家族成员具有较高的同源性,通过PROSITE的ScanProsite软件分析得出,该蛋白质含有1个trypsindomain(第32-264位),在NCBI的CDD数据库中分析结果显示该酶是1种胰蛋白酶样丝氨酸蛋白酶。更多还原

【Abstract】 Four compounds of Urechis unicinctus fibrinolytic enzymes(UFE) with different molecular weight were isolated and purified by our laboratory.Each of them not only improves the secondary plasmin activity of the organisms but also has great anticoagulant activity,plasmin activity and histocompatibility.According to the N-terminal amino acid sequence of the UFEⅡ,degenerate primers were designed to amplify part of the UFEⅡ gene by using cDNA as a template through 3 ’-RACE PCR.Then a 906bp length complete cDNA sequence which encoding the UFEⅡwas amplyfied on the basis of the result of 5’-RACE PCR.The comparison result of this cDNA sequence analyzed by BLASTx indicated that the product of it had high homology with the tryptase family members.The analysis results of ScanProsite software by PROSITE demonstrated that the protein contains a trypsin domain(No.32-264 bit) and according to the CDD database of NCBI,the protein is a trypsin-like serine protease.更多还原