【摘要】 选择蒽、9-甲基蒽和9,10-二甲基蒽作为模型化合物,以李村河口区沉积物为培养基质,进行实验室好氧微生态培养,通过超声萃取-柱层析-气相色谱法测定目标污染物浓度随培养时间的变化,研究了它们在自然菌群作用下的生物降解行为。测定方法的基质加标回收率在66.20%~82.95%之间,相对标准偏差在3.34%~6.19%之间。6个月的好氧生物降解实验表明,沉积物中本底蒽由32.88 ng.g-1降到32.32 ng.g-1,降解率仅为1.70%;而在添加了蒽的沉积物样品中其浓度由170.40 ng.g-1降到151.36 ng.g-1,降解率高达11.17%;加入的9-甲基蒽和9,10-二甲基蒽的浓度也分别由133.84 ng.g-1和144.26 ng.g-1减少至122.52 ng.g-1和135.13 ng.g-1,降解率分别达到8.46%和6.33%。相同条件下三种有机污染物的不同降解速率表明,9,10位取代使得蒽在沉积环境中更加持久,自然降解速率更慢更多还原

【Abstract】 Anthracene(AC),9-methyl anthracene(9-MA) and 9,10-dimethyl anthracene(9,10-DMA) were selected as model compounds,and LiCun esturary sediment was selected as incubation substrate.The concentration change of three targeted pollutants were determined by a method using ultrasonic extraction-column chromatography-gas chromatography.The biodegradation of these three model compounds in a labotory microcosm system were investigated.The recoveries of three targeted pollutants for spiked matrics were 66.20%~82.95%,with RSD ranged from 3.34% to 6.19%.A 6-month aerobic incubation experiment showed that the concentration of AC decreased from 32.88 ng·g-1 to 32.32 ng·g-1 in the control experiment,corresponding to a degradation percentage of 1.70%.However,the degradation percentage of the spiked AC was 11.17%,with concentration reduced from 170.40 ng·g-1 to 151.36 ng·g-1.For spiked 9-MA and 9,10-DMA samples,the concentration were declined from the initial values of 133.84 ng·g-1 and 144.26 ng·g-1 to 122.52 ng·g-1 and 135.13 ng·g-1,respectively,accounting for degradation percentages of 8.46% and 6.33% separately.Different degradation percentages of the three targeted pollutants under the same condition indicated that methyl-substitution resulted in higher persistence of AC in sedimentary environment with lower natural attenuation rate.更多还原