【摘要】 目的研究Livin在急性白血病中的表达,探讨其临床意义。方法采用半定量逆转录—聚合酶链反应(RT-PCR)检测40例急性白血病(AL)患者(AL组)LivinmRNA的表达水平,其中初治患者16例(初治亚组)、复发患者11例(复发亚组)、完全缓解患者13例(缓解亚组)。另选10例健康人(对照组)的标本作正常对照。结果初治亚组中LivinmRNA的表达较对照组明显升高(P<0.05),治疗缓解后的表达水平下降(P<0.05),复发时又再次升高;初治亚组Livin阳性的患者缓解率(14.3%)低于表达阴性的患者(77.8%)(P<0.05)。LivinmRNA的表达与AL患者性别、年龄无关(P>0.05)。结论 Livin基因的过度表达参与了AL的发病,并且是AL复发的高危因素。Livin基因的高表达是判断AL预后不良的指标之一。更多还原

【Abstract】 Objective To research the expression of Livin in acute leukemia,and explore its clinical significance.Methods The expression of Livin mRNA of 40 cases patients with acute leukemia(AL) was measured by semi-quantity reverse transcription polymers chain reaction(RT-PCR).In which,16 cases newly diagnosed patients(newly diagnosed sub-group),11 cases patients relapsed(recurrence sub-group),13 cases patients with complete remission(remission sub-group).Another 10 healthy people were as control group.Results The expression of Livin in newly diagnosed sub-group was higher than that in control group(P<0.05),while it decreased in patients with remission sub-group(P<0.05);In relapsed patients,the expression of Livin increased again.The response rate of patients with Livin positive(14.3%) was lower than that of patients with Livin negative(77.8%) in newly diagnosed sub-group(P<0.05).The expression of Livin mRNA in patients with AL had nothing to do with sex and age(P>0.05).Conclusion Overexpression of Livin may play a synergic role in the pathogenesis of leukemia and may be a high risk factor for relapse in AL.High expression of Livin can serve as one of the markers of poor prognosis in AL.更多还原