【摘要】 为了建立检测绵羊口蹄疫病毒(Footand mouth disease virus,FMDV)整联蛋白受体αv亚基基因mRNA相对表达量的荧光定量RT-PCR方法,本研究根据报道的绵羊FMDV整联蛋白受体αv亚基(integrin,alphaV,ITGAV)基因序列设计实时定量PCR引物,以β-actin为内参基因,采用相对荧光定量RT-PCR方法,检测分析αv基因在绵羊体内不同组织器官中的mRNA表达谱。结果显示αv基因在绵羊19种组织中均有不同程度的转录表达,在乳腺组织中表达量最高,蹄组织次之,肌肉组织最少,卵巢、瘤胃、气管、小肠、肺等组织上也有不同程度的表达。本研究成功建立了检测FMDV整联蛋白受体αv亚基基因在绵羊不同器官组织中mRNA表达水平的相对荧光定量RT-PCR方法,并明确了αv基因在不同组织间的表达差异,为FMDV组织嗜性研究及分子生物学检测方法的建立提供了资料。更多还原

【Abstract】 For establishing an assay of Real-time PCR to detect distribution and expression level of sheep Food and mouth disease virus(FMDV) integrin receptor αv submit in different tissue,in this study,a pair of primers was designed according to the published nudeotide sequence of integrin receptor αv submit.And tissue expression profile of sheep(Ovisaries) integrin αv in different tissue was detected by Real-time PCR.The results showed that the integrin receptor αv submit was generally expressed in 19 kinds of tissues with different level.The highest expression level was showed in breast,the next was in hoof,and the lowest was in muscle tissue.We have established the Real-time PCR method which can be used to confirm tissue profile of sheep FMDV integrin receptor αv submit.This study provides basic data for further study tissue tropism of FMDV and molecular detection method.更多还原