【摘要】 蜀恢527(Oryza sativa)因其一般配合力高、所配组合杂种优势强、衍生恢复系多等优点,被认为是现阶段杂交水稻育种的骨干亲本之一。本研究通过亲本性状遗传规律分析,结合全基因组扫描,拟阐明蜀恢527的遗传组成,确定其产量相关性状的关键基因组区域。系谱产量相关性状分析结果显示,结实率高低,每穗实粒数、每穗总粒数和有效穗的多少可能源自IR24-蜀恢527的遗传途径;而千粒重、单株产量的高低和有效穗的多少则可能源自圭630-R1318-蜀恢527的遗传途径。采用1050个SSR引物对相关亲本进行全基因组扫描,构建了蜀恢527基因组来源图谱。分析发现,所有品种共有(无多态性标记)的区段占62.94%;约有17.53%的区段可能来源于多个亲本(多态性标记不足以区分各亲本);在蜀恢527形成过程中,R1318贡献了13.68%区段,辐36-2贡献了0.53%的区段,IR24贡献了1.32%的区段;同时,蜀恢527基因组内包含了4%的自身特有区段。根据性状遗传途径和基因组来源图谱,初步确定了蜀恢527关键基因组区域。整合文献报道有关产量的QTL定位结果,发现了一些可能解释关键区段影响产量的候选QTL位点。本研究对于骨干亲本选育和超级杂交稻育种具有重要意义。更多还原

【Abstract】 As one of the backbone parents of hybrid rice breeding at present,Shuhui 527(Oryza sativa) has many merits,such as high general combining ability,strong heterosis of hybrid combination,and many derivative R-lines.In order to clarify the genetic composition and the key genome regions related yield characters of Shuhui 527,the heredity and the entire genome of Shuhui 527 were researched in this study.The results showed that seed-setting rate,filled grain number per panicle,grain number per panicle and panicles were possibly derived from the pedigree of IR24-Shuhui527;the weight of a thousand seeds,plant weight and panicles were possibly derived from the pedigree of Gui630-R1318-Shuhui 527.The genomic map of Shuhui 527 and its related ancestral parents were constructed by scanning the entire genome with 1050 SSR primers,the results revealed that(1) the shared fragments of all varieties(i.e.none polymorphic fragments) accounted for 62.94%;(2) putative multi-originated fragments(polymorphic marker was unable to identify its origin) accounted for approximately 17.53%;(3) in the breeding process of Shuhui 527,R1318 had contributed 13.68% fragments,Fu36-2 had contributed 0.53% fragments,and IR24 had contributed 1.32% fragments;(4) Shuhui 527 had contributed 4% of itself fragments.The results indicated that the key genome regions of Shuhui 527 is initially determined,and some influencing output candidate QTL position spots of key fragments are found according to the analysis of the results of reported yield QTLs.The present study will benifit to the core parent development and supper hybid rice breeding.更多还原