【摘要】 为探讨生物传感器在纤维素乙醇测定中的应用,采用乙醇生物传感器分析仪对发酵液中的纤维素乙醇含量进行测定,分析其特点、规律。结果:乙醇生物传感分析仪线性关系良好,线性范围为0.2～20.0mg/100mL,回归方程为:Y=5.00225X+0.72273,R=0.99976;对乙醇的检测快速而准确,检测时间为20s,回收率为99.3%～100.4%;与气相色谱方法、酒精计法相比较,差异不显著(P>0.05);专一性强,且仪器重现性和稳定性较好,检测灵敏度高,检测下限为0.2mg/100mL。乙醇生物传感分析仪用于纤维素乙醇检测,具有检测精度高、速度快(20s)、稳定性好、使用成本低等优势,有望代替酒精计、气相色谱仪等,成为检测纤维素乙醇含量的新方法、新手段。更多还原

【Abstract】 In order to estimate the application in the cellulose ethanol production by fermentation,an ethanol biosensor was used for cellulose ethanol in the broth during the ethanol fermentation process and the characteristics and patterns were investigated in this study.It was found that the results detected by the biosensor showed good linear relationship within the scope of 0.2-20.0 mg/100 mL,and the regression equation was y=5.00225x+0.72273(R=0.99976).The detector could detect the ethanol rapidly and accurately,and the response time was 20s,recovery ratio was of 99.3%-100.4%.The results showed no significant differences(P>0.05) between the detector and the traditional methods(i.e.gas chromatography and alcoholmeter).And the detector also showed some good merits such as high specificity,good repeatability,high precision(the detection lower limit of 0.2 mg/ml),high speed and stable.In this study,it could be concluded that the ethanol biosensor could be used for cellulosic ethanol determination,and it might replace the main methods(gas chromatography and alcoholmeter) for ethanol detection and would become the novel method for cellulosic ethanol estimation.更多还原