【摘要】 为提高虾壳的综合利用效果,该文以虾壳为原料,将高电压脉冲电场(PEF)技术用于壳聚糖的提取研究。与常规加热法和微波法进行了对比试验,并通过单因素试验和三元二次回归旋转组合设计确定最佳制备工艺。结果表明:该技术与传统加热和微波法方法相比,具有非热、反应速度快,脱乙酰度高的特点。建立了脉冲数、场强、NaOH质量浓度对脱乙酰度影响的数学模型,确定了最佳工艺参数:脉冲数10,电场强度20.48kV/cm,NaOH质量分数48.64%,该条件下脱乙酰度达到最大92.32%。因此,利用高电压脉冲电场技术可以快速制备虾壳中的壳聚糖。更多还原

【Abstract】 In order to improve the comprehensive utilization of shrimp shells,chitosan of shrimp shell was extracted by the high intensity pulsed electric fields (PEF).Optimal processing parameters were acquired by the single-factor experiment and quadratic regression rotation design,which were also compared with that of heating method and microwave method.It was found that the PEF method has the features of non-thermal,fast response and high deacetylation compared with traditional methods.The mathematical model of the field strength,pulse number and the concentration of sodium hydroxide on the degree of deacetylation was established,and the optimum condition was that: pulse number 10,field strength 20.48 kV/cm,the concentration of sodium hydroxide 48.64%.Under the optimum condition,the degree of deacetylation reached up to 92.32%.So PEF could be a quick method to prepare chitosan from shrimp shell.更多还原