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抗堆型艾美球虫子孢子单克隆抗体杂交瘤细胞的复苏及其活性的初步鉴定

Recovery and Initial Identification of the Hybridoma Cells Secreting McAbs Against Sporozoites of E.acervulina

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【Author】 KANG Gui-ying1,QIN Jian-hua2,ZHAI Li-juan1,ZHONG Gang3,ZHAO Jing-wen1,Meihua1(1.College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028000,China;2.College of Animal Science and Technology,Hebei Agriculture University,Baoding 071001,China;3.Inner Mongolia Livestock Improvement Station,Huhhot 010011,China)

【机构】内蒙古民族大学动物科技学院；河北农业大学动物科技学院；内蒙古家畜改良工作站；

【摘要】将堆型艾美耳球虫卵囊孢子化、破碎、脱去孢子囊、纯化子孢子并将其超声裂解,最终离心得到子孢子可溶性抗原,经紫外分光光度法测得抗原样品的浓度为1.58mg/mL.将保存的杂交瘤细胞解冻,培养并利用其制备抗体.用制备的抗原对此抗体进行了初步鉴定.更多还原

【Abstract】 E.acervulina oocysts were rejuvenated,purificated and sporulated,sporozoites from broken sporangium were purificated and sonicated,Soluble antigen of Plasmodium was obtained from Ultrasonic degradation products.concentration of antigen samples measured by UV spectrophotometry is 1.58mg/mL.The hybridoma cell was thawed,cultured,Antibodies were pr-epared using hybridoma cell and identified with soluble antigen.更多还原

【关键词】堆型艾美耳球虫；杂交瘤细胞；子孢子；
【Key words】 E.acervulina； Hybridoma Cell； Sporozoite；

【文献出处】内蒙古民族大学学报(自然科学版) ,Journal of Inner Mongolia University for Nationalities(Natural Sciences) , 编辑部邮箱 ,2011年01期

【分类号】S852.7
【被引频次】2
【下载频次】85

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