【摘要】 目的:研究弱精子症特异表达基因,加深对弱精子症发生分子机制的认识。方法:采用GATHER、PANTHER以及ToppGene在线生物信息学分析工具,对弱精子症差异表达基因进行生物信息学分析挖掘。结果:通过上述生物信息学软件分析发现,弱精子症差异表达基因在细胞蛋白质及大分子生物代谢过程、蛋白修饰、细胞死亡、细胞凋亡以及凋亡介导中发挥着重要作用。结论:通过生物信息学挖掘发现弱精子症患者精子在活力下降的同时,精子的基本生命活动也下降,同时更易于发生凋亡或死亡。参与细胞骨架构成、微管运动、细胞运动的一些差异表达基因,如KIF3B、MYO15A、KIF6、KIF26B、KIF3A、DNHD2、DMN、DYNC2H1、STARD9、MYOHD1、TPM1等,可能与弱精子症相关,值得进一步深入研究。更多还原

【Abstract】 Objective: To study the differentially expressed genes in asthenospermia to gain a deeper insight into the molecular mechanisms of the disease.Methods: We analyzed the differentially expressed genes in asthenospermia using GATHER,PANTHER and ToppGene online bioinformatics tools.Results: Our bioinformatics mining and analyses revealed that the differentially expressed genes in asthenospermia played important roles in the cellular protein and macromolecular metabolism,protein modification,cell death,cell apoptosis and apoptosis induction.Conclusion: Asthenospermia patients experience a decline in sperm activity and the basic life activities of sperm simultaneously,and are also prone to cell apoptosis or death.Such differentially expressed genes as KIF3B,MYO15A,KIF6,KIF26B,KIF3A,DNHD2,DMN,DYNC2H1,STARD9,MYOHD1,and TPM1,which are involved in cytoskeletal structure,microtubule movement and cell movement,may be associated with asthenospermia,and therefore deserve further studies.更多还原