【摘要】 目的:探讨弱精子症患者精子中富含半胱氨酸的分泌蛋白2(CRISP2)mRNA及蛋白的表达水平,探讨其与精子活力的关系及相关的分子机制。方法:收集成年男性弱精子症患者精液78例,活力正常精液70例作为对照组,50% Percoll离心分离纯化后,提取精子总RNA及总蛋白,采用RT-PCR,SYBR Green实时定量PCR和Western印迹技术检测CRISP2 mRNA及蛋白相对表达量。结果:CRISP2基因在弱精子症患者精子中的表达量明显低于其在正常对照组精子中的表达量,下降达4.3倍;Western印迹发现CRISP2蛋白在弱精子症组较正常对照组下降达1.71倍,两组之间的表达量有明显的统计学差异(P<0.05)。结论:CRISP2基因及蛋白在弱精子症患者精子中的表达下调可能与精子活力下降有密切联系,提示CRISP2基因及蛋白可能成为弱精子症发病机制研究的一个分子靶标。更多还原

【Abstract】 Objective:To investigate the mRNA and protein expression levels of cysteine-rich secretory protein 2(CRISP2) in the sperm of asthenospermia patients,and explore their relationship with sperm motility and related molecular mechanism.Methods:We collected 78 semen samples from adult male patients with asthenospermia and another 70 from healthy volunteers as controls.We extracted total RNA and total protein from the sperm following purification of the sperm by Percoll gradient centrifugation,and detected the relative expressions of CRISP2 mRNA and protein in the two groups by RT-PCR,SYBR Green real-time PCR and Western blot.Results:The expression of CRISP2 mRNA was down-regulated by 4.3 times and that of the CRISP2 protein by 1.71 times in the asthenospermia patients,significantly lower than in the normal control group(P<0.05).Conclusion:The down-regulation of CRISP2 mRNA and protein expressions in the sperm of asthenospermia patients may be closely related with decreased sperm motility,which suggests that CRISP2 may serve as a potential molecular target for the research of asthenospermia.更多还原