【摘要】 研究了椰汁对4个大花蕙兰杂交系无菌播种超含水态苗发生的影响,发现椰汁是引起大花蕙兰小植株超含水态发生的重要因素.椰汁用量不同,不同杂交组合超含水态发生情况差异较大,最高发生率达到37.0%,最低为零.椰汁对超含水态的发生具有重要影响.当激素6-BA和IBA用量一定时,在3.0%～6.0%范围内,随着椰汁用量的增加,超含水态苗数量升高,超含水态发生指数最高达到26.0.分析表明,椰汁中含有的激素、糖类和氨基酸,引起培养基中激素、糖类和铵离子浓度的增加,进而引起超含水态现象.更多还原

【Abstract】 Chose 4 hybrid groups of Cymbidium,and explored the effects of coconut milk on hyperhydricity of Cymbidium plantlet in vitro.Reveals firstly that coconut milk is an important factor causing hyperhydricity of Cymbidium plantlet.It showed that different hybrid groups differed in hyperhydricity occurrence with the content variation of coconut milk.The highest rate reached 37.0%,while the lowest one was zero.Coconut milk has great effects on the occurrence of hyperhydricity.When the quantity of 6-BA and IBA were fixed,the number of plantlets infected by hyperhydricity increased with the increasing of coconut milk within 3.0%～6.0%,in which the highest hyperhydricity index got 26.0.We realize that the hormone, sugars,and amino acids contained in coconut milk raised the level of hormone,sugars, and NH concentrations in the culture media,and resulted high hyperhydricity occurrence.The paper created the idea of hyperhydricity index which evaluates the occurrence of hyperhydricity, and get accurate and better appraisement.更多还原